Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 3, Problem 28P
Interpretation Introduction
Interpretation:
In the given experiment, the observation needs to be explained, if the given protein does not contain any disulfide bond and if it contains a single disulfide bond. An experiment to identify the cysteine residue containing the disulfide bond needs to be explained.
Concept introduction:
2-D electrophoresis is a technique that is used to analyze proteins. Here mixture of proteins is separated based on two properties in two dimensions of the same gel. Normally, in the first dimension proteins are separated according to the isoelectric point and then the proteins are separated in a perpendicular dimension based on their mass.
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Movement of Dyes in an Agarose Gel.This problem tests your ability to understand basic concepts of agarose gel electrophoresis.The gel is loaded from lane 1 at the top to lane 7 at the bottom with: bromophenol blue, safranin O, orange G, crystal violet, xylene cyanol, methylene blue, and the unknown.Lines have been added to the gel to help you locate the center of the wells.
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Affinity purification: Assemble the following components to set up affinity chromatography to purify a protein.
Which protein do
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Glutathione
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Troubleshooting gels. What effect will be observed on the stained bands of protein in apolyacrylamide gel if…a. …the samples are not incubated at 95˚C prior to loading?b. …the gel is run at too high or too low a voltage?c. …too much protein is loaded?
Chapter 3 Solutions
Biochemistry
Ch. 3 - Prob. 1PCh. 3 - Prob. 2PCh. 3 - Prob. 3PCh. 3 - Prob. 4PCh. 3 - Prob. 5PCh. 3 - Prob. 6PCh. 3 - Prob. 7PCh. 3 - Prob. 8PCh. 3 - Prob. 9PCh. 3 - Prob. 10P
Ch. 3 - Prob. 11PCh. 3 - Prob. 12PCh. 3 - Prob. 13PCh. 3 - Prob. 14PCh. 3 - Prob. 15PCh. 3 - Prob. 16PCh. 3 - Prob. 17PCh. 3 - Prob. 18PCh. 3 - Prob. 19PCh. 3 - Prob. 20PCh. 3 - Prob. 21PCh. 3 - Prob. 22PCh. 3 - Prob. 23PCh. 3 - Prob. 24PCh. 3 - Prob. 25PCh. 3 - Prob. 26PCh. 3 - Prob. 27PCh. 3 - Prob. 28PCh. 3 - Prob. 29PCh. 3 - Prob. 30PCh. 3 - Prob. 31P
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