(a) Interpretation: The digestion of octapeptide AVGWRVKS with trypsin. The most suitable method for separation of the products between ion-exchange or gel-filtration chromatography should be determined. Concept introduction: A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

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Answer 1

Question

Chapter 3, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The digestion of octapeptide AVGWRVKS with trypsin. The most suitable method for separation of the products between ion-exchange or gel-filtration chromatography should be determined.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

Interpretation Introduction

(b)

Interpretation:

If the protein was digested with chymotrypsin. The optimal separation method should be explained.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

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a. An oligopeptide ALVGALGATPTPQMWSHSWRGVSIKS was digested with trypsin.Which method would be most appropriate for separating the products: ion exchange or gel filtration chromatography? Explain.b. Suppose that the peptide was digested with cyanogen bromide. What would be the optimal separation technique? Explain

Wh are doing this procedures can you explain? (ex. heating or adding chemicals etc.) 1) Purification of Vitelline from Egg Yolk-Experimental ProcedureMeasure the volume of 3 egg yolks and mix by adding an equal volume of NaCl solution.Extract the mixture with 3-fold volumes of ether and separate the aqueous phase.Do the same procedure 3 times.Mix the sample with water and rinse.It is expected for the protein to collapse.Some more water is added in order to check whether the collapse occurs completely.The sample is centrifuged and the precipitate is dried. 2) Purification of Plasma Albumin and Fibrinogen-Experimental ProcedureAdd ammonium sulfate to 10 ml plasma up to 25% saturation.Separate the collapsed fibrinogen by centrifuge.Increase the saturation level to 33% by adding (NH4) 2SO4.Separate the globulin by centrifugation.Separate prodoglobulins by increasing the saturation level to 46%.Increase the saturation level up to 64% to precipitate albumin.Separate the albumin by centrifuge…

topic: Bradford AssayThere are numerous methods of protein determination in use, but this module focuses on the Bradford assay.The Bradford assay is a dye-binding method that employs Coomassie Brilliant Blue G-250, whose structureis shown in Figure 2.3.4.1. Coomassie Brilliant Blue G-250 is a dye that interacts with proteins throughhydrophobic and electrostatic interactions. What are the identities and functions of the components of the Bradford reagent in protein contentdetermination?

Answer 2

Question

Chapter 3, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The digestion of octapeptide AVGWRVKS with trypsin. The most suitable method for separation of the products between ion-exchange or gel-filtration chromatography should be determined.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

Interpretation Introduction

(b)

Interpretation:

If the protein was digested with chymotrypsin. The optimal separation method should be explained.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

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Answer 3

Question

Chapter 3, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The digestion of octapeptide AVGWRVKS with trypsin. The most suitable method for separation of the products between ion-exchange or gel-filtration chromatography should be determined.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

Interpretation Introduction

(b)

Interpretation:

If the protein was digested with chymotrypsin. The optimal separation method should be explained.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

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Answer 4

Question

Chapter 3, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The digestion of octapeptide AVGWRVKS with trypsin. The most suitable method for separation of the products between ion-exchange or gel-filtration chromatography should be determined.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

Interpretation Introduction

(b)

Interpretation:

If the protein was digested with chymotrypsin. The optimal separation method should be explained.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

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Answer 5

Chapter 3, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The digestion of octapeptide AVGWRVKS with trypsin. The most suitable method for separation of the products between ion-exchange or gel-filtration chromatography should be determined.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

Interpretation Introduction

(b)

Interpretation:

If the protein was digested with chymotrypsin. The optimal separation method should be explained.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

Answer 6

Chapter 3, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The digestion of octapeptide AVGWRVKS with trypsin. The most suitable method for separation of the products between ion-exchange or gel-filtration chromatography should be determined.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

Answer 7

Chapter 3, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The digestion of octapeptide AVGWRVKS with trypsin. The most suitable method for separation of the products between ion-exchange or gel-filtration chromatography should be determined.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

Answer 8

Interpretation Introduction

(b)

Interpretation:

If the protein was digested with chymotrypsin. The optimal separation method should be explained.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

Answer 9

Interpretation Introduction

(b)

Interpretation:

If the protein was digested with chymotrypsin. The optimal separation method should be explained.

Concept introduction:

A peptide bond is present between the two amino acids in a protein. During the formation of a peptide bond, a molecule of water is released. The amino group of an amino acid gets associated with the carboxyl group of another. Polypeptides and proteins are the chains formed by the amino acids.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 3 - Prob. 1P Ch. 3 - Prob. 2P Ch. 3 - Prob. 3P Ch. 3 - Prob. 4P Ch. 3 - Prob. 5P Ch. 3 - Prob. 6P Ch. 3 - Prob. 7P Ch. 3 - Prob. 8P Ch. 3 - Prob. 9P Ch. 3 - Prob. 10P

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