(a) Interpretation: The products obtained from the reaction of the peptide of hormone glucagon and trypsin at pH 8 is to be stated. Concept introduction: Trypsin is an enzyme that is found in the digestive system where it hydrolyzes the protein. It is also used to cleave the peptide chain at the carboxyl end of lysine and arginine. It does not cleave the peptide chain if amino acid proline followed by arginine and lysine is present.

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Answer 1

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Definition Definition Organ system that helps in the digestion of food by mechanical and biochemical processes. The digestive system breaks down food into simpler components that enable the body to then absorb or transport nutrients. The human digestive system includes the gastrointestinal tract or alimentary canal and the associate glands. The organs of the gastrointestinal tract include the mouth, pharynx, esophagus, stomach, small intestine, and large intestine. The associated glands include salivary glands, liver, gallbladder, and pancreas.

Chapter 27, Problem 27.52AP

Interpretation Introduction

(a)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and trypsin at pH 8 is to be stated.

Concept introduction:

Trypsin is an enzyme that is found in the digestive system where it hydrolyzes the protein. It is also used to cleave the peptide chain at the carboxyl end of lysine and arginine. It does not cleave the peptide chain if amino acid proline followed by arginine and lysine is present.

Interpretation Introduction

(b)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and Ph—N=C=S, CF3CO2H then the aqueous solution is to be stated.

Concept introduction:

Edman degradation is a method of peptide sequencing. In this method, N− terminal of the peptide chain is treated with phenyl isothiocyanate which is known as Edman reagent. It results in the separation of that amino-terminal residue without affecting the rest peptide chain.

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Vasopressin is a peptide hormone synthesized by the hypothalamus; in its reduced form it has the structure shown. NH2 HS H H H H H *H3N° NH2 N' SH NH2 ОН Vasopressin NH *H2N= NH2 This structure is incorrect in that one of the amino acids is shown in the D-configuration, rather than the L-. Which one is it? What is the amino acid sequence of this peptide? Enter your answer using the single letter abbretiavions for the amino acids; i.e. GPT, not Gly-Pro-Thr. What is the net charge of this peptide at physiological pH? ZI IZ ZI IZ ZI

Treatment of a polypeptide with 2-mercaptoethanol yields two polypeptides with the following primary structures: Val-Met-Tyr-Ala-Cys-Ser-Phe-Ala-Glu-Ser Ser-Cys-Phe-Lys-Cys-Trp-Lys-Tyr-Cys-Phe-Arg-Cys-Ser Treatment of the original intact polypeptide with chymotrypsin yields the following peptides: 1. Ala, Glu, Ser 3. Tyr, Val, Met 5. Ser, Phe, 2 Cys, Lys, Ala, Trp 2. 2 Phe, 2 Cys, Ser 4. Arg, Ser, Cys 6 Tyr, Lys Determine the positions of the disulfide bridges in the original polypeptide.

considering the following peptides: Phe-Tyr-Ala-Lys-Glu-Asp the amino acid constituents that will have a protonated amino group at pH 7 is: a- lys and asp only b-phe, lys, and asp only c- phe and lys only d- lys only

Answer 2

Question

Definition Definition Organ system that helps in the digestion of food by mechanical and biochemical processes. The digestive system breaks down food into simpler components that enable the body to then absorb or transport nutrients. The human digestive system includes the gastrointestinal tract or alimentary canal and the associate glands. The organs of the gastrointestinal tract include the mouth, pharynx, esophagus, stomach, small intestine, and large intestine. The associated glands include salivary glands, liver, gallbladder, and pancreas.

Chapter 27, Problem 27.52AP

Interpretation Introduction

(a)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and trypsin at pH 8 is to be stated.

Concept introduction:

Trypsin is an enzyme that is found in the digestive system where it hydrolyzes the protein. It is also used to cleave the peptide chain at the carboxyl end of lysine and arginine. It does not cleave the peptide chain if amino acid proline followed by arginine and lysine is present.

Interpretation Introduction

(b)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and Ph—N=C=S, CF3CO2H then the aqueous solution is to be stated.

Concept introduction:

Edman degradation is a method of peptide sequencing. In this method, N− terminal of the peptide chain is treated with phenyl isothiocyanate which is known as Edman reagent. It results in the separation of that amino-terminal residue without affecting the rest peptide chain.

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Answer 3

Question

Definition Definition Organ system that helps in the digestion of food by mechanical and biochemical processes. The digestive system breaks down food into simpler components that enable the body to then absorb or transport nutrients. The human digestive system includes the gastrointestinal tract or alimentary canal and the associate glands. The organs of the gastrointestinal tract include the mouth, pharynx, esophagus, stomach, small intestine, and large intestine. The associated glands include salivary glands, liver, gallbladder, and pancreas.

Chapter 27, Problem 27.52AP

Interpretation Introduction

(a)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and trypsin at pH 8 is to be stated.

Concept introduction:

Trypsin is an enzyme that is found in the digestive system where it hydrolyzes the protein. It is also used to cleave the peptide chain at the carboxyl end of lysine and arginine. It does not cleave the peptide chain if amino acid proline followed by arginine and lysine is present.

Interpretation Introduction

(b)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and Ph—N=C=S, CF3CO2H then the aqueous solution is to be stated.

Concept introduction:

Edman degradation is a method of peptide sequencing. In this method, N− terminal of the peptide chain is treated with phenyl isothiocyanate which is known as Edman reagent. It results in the separation of that amino-terminal residue without affecting the rest peptide chain.

Expert Solution & Answer

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Answer 4

Question

Definition Definition Organ system that helps in the digestion of food by mechanical and biochemical processes. The digestive system breaks down food into simpler components that enable the body to then absorb or transport nutrients. The human digestive system includes the gastrointestinal tract or alimentary canal and the associate glands. The organs of the gastrointestinal tract include the mouth, pharynx, esophagus, stomach, small intestine, and large intestine. The associated glands include salivary glands, liver, gallbladder, and pancreas.

Chapter 27, Problem 27.52AP

Interpretation Introduction

(a)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and trypsin at pH 8 is to be stated.

Concept introduction:

Trypsin is an enzyme that is found in the digestive system where it hydrolyzes the protein. It is also used to cleave the peptide chain at the carboxyl end of lysine and arginine. It does not cleave the peptide chain if amino acid proline followed by arginine and lysine is present.

Interpretation Introduction

(b)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and Ph—N=C=S, CF3CO2H then the aqueous solution is to be stated.

Concept introduction:

Edman degradation is a method of peptide sequencing. In this method, N− terminal of the peptide chain is treated with phenyl isothiocyanate which is known as Edman reagent. It results in the separation of that amino-terminal residue without affecting the rest peptide chain.

Expert Solution & Answer

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Answer 5

Chapter 27, Problem 27.52AP

Interpretation Introduction

(a)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and trypsin at pH 8 is to be stated.

Concept introduction:

Trypsin is an enzyme that is found in the digestive system where it hydrolyzes the protein. It is also used to cleave the peptide chain at the carboxyl end of lysine and arginine. It does not cleave the peptide chain if amino acid proline followed by arginine and lysine is present.

Interpretation Introduction

(b)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and Ph—N=C=S, CF3CO2H then the aqueous solution is to be stated.

Concept introduction:

Edman degradation is a method of peptide sequencing. In this method, N− terminal of the peptide chain is treated with phenyl isothiocyanate which is known as Edman reagent. It results in the separation of that amino-terminal residue without affecting the rest peptide chain.

Answer 6

Chapter 27, Problem 27.52AP

Interpretation Introduction

(a)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and trypsin at pH 8 is to be stated.

Concept introduction:

Trypsin is an enzyme that is found in the digestive system where it hydrolyzes the protein. It is also used to cleave the peptide chain at the carboxyl end of lysine and arginine. It does not cleave the peptide chain if amino acid proline followed by arginine and lysine is present.

Answer 7

Chapter 27, Problem 27.52AP

Interpretation Introduction

(a)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and trypsin at pH 8 is to be stated.

Concept introduction:

Trypsin is an enzyme that is found in the digestive system where it hydrolyzes the protein. It is also used to cleave the peptide chain at the carboxyl end of lysine and arginine. It does not cleave the peptide chain if amino acid proline followed by arginine and lysine is present.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and Ph—N=C=S, CF3CO2H then the aqueous solution is to be stated.

Concept introduction:

Edman degradation is a method of peptide sequencing. In this method, N− terminal of the peptide chain is treated with phenyl isothiocyanate which is known as Edman reagent. It results in the separation of that amino-terminal residue without affecting the rest peptide chain.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The products obtained from the reaction of the peptide of hormone glucagon and Ph—N=C=S, CF3CO2H then the aqueous solution is to be stated.

Concept introduction:

Edman degradation is a method of peptide sequencing. In this method, N− terminal of the peptide chain is treated with phenyl isothiocyanate which is known as Edman reagent. It results in the separation of that amino-terminal residue without affecting the rest peptide chain.

Answer 10

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Answer 12

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Answer 13

Ch. 27 - Prob. 27.1P Ch. 27 - Prob. 27.2P Ch. 27 - Prob. 27.3P Ch. 27 - Prob. 27.4P Ch. 27 - Prob. 27.5P Ch. 27 - Prob. 27.6P Ch. 27 - Prob. 27.8P Ch. 27 - Prob. 27.9P Ch. 27 - Prob. 27.10P Ch. 27 - Prob. 27.11P

Solution Summary: The author explains that trypsin cleaves the amino acid sequence where lysine and arginine amino acids are present.

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