BIOLOGY
BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
bartleby

Concept explainers

bartleby

Videos

Textbook Question
Book Icon
Chapter 27, Problem 1DA

Data analysis If the excision of an F plasmid Is not precise, and some f coli DNA is added to the F plasmid, what are the consequences when this plasmid is transferred to a new cell?

Expert Solution & Answer
Check Mark
Summary Introduction

To determine: The consequences of plasmid transference if the plasmid excision is not precise.

Introduction: The exchange of F plasmid takes place from the F+, which is a donor, to the F- that acts as the recipient. The F factor is transferred by the process called conjugation. The F plasmid has genes that are required for conjugation as well as infer some additional properties to the bacteria.

Explanation of Solution

The plasmid and the fragments of the genetic material can be excised with the help of specific enzymes known as restriction nucleases. These can be endonuclease or exonuclease. The plasmids need to be excised precisely so that the sticky ends or the complementary sequence ends can bind with the genetic material of the host organisms.

If the plasmid is not cut precisely then the frequency of the plasmids to combine with the genetic material of the bacteria (E.coli) will be reduced significantly. The few plasmids that have joined themselves to the bacterial genome will be very difficult to identify as the boundaries of the plasmid and the host genome will not be accurately known. To prevent such errors the endonuclease that makes precise cuts are preferred for cutting the plasmid and the host’s DNA.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!
Students have asked these similar questions
draw structure of plasmid
Preforming a "blue-white screen" 3) Would bacteria that have taken up a plasmid into which a DNA fragment has been inserted, form a blue colony or a white colony when grown on this medium? Briefly explain why these bacteria would form a colony of the color you chose.
help me with this multiple choice question please, this is molecular and cell biology Visualization of an electrophoresed agarose gel (D) Plasmid DNA cannot be visualized on an agarose gel (A) Plasmid DNA may be visualized as multiple supercoiled forms (B) Visualized plasmid DNA is always linear Ⓒ(C) Visualized plasmid DNA is always circular
Knowledge Booster
Background pattern image
Biology
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
SEE MORE QUESTIONS
Recommended textbooks for you
Text book image
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Text book image
Biology: The Unity and Diversity of Life (MindTap...
Biology
ISBN:9781305073951
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Bacterial Genomics and Metagenomics; Author: Quadram Institute;https://www.youtube.com/watch?v=_6IdVTAFXoU;License: Standard youtube license