BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 26, Problem 5A
Summary Introduction
Introduction: The study of microbes in the natural living environment that involves complex microbial communities in which they exist is called metagenomics. This study examines the genomic composition of microorganisms.
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In sequential order, what are the three steps of PCR?
A.
Denature DNA, Anneal Primers, Extend DNA
B.
Denature DNA, Extend DNA, Anneal Primers
C.
Anneal Primers, Extend DNA, Denature DNA
D.
Extend DNA, Anneal Primers, Denature DNA
Which gene is incorporated into plasmids to detect recombinantcells?a. restriction endonuclease
b. virus receptors
c. a gene for antibiotic resistance
d. reverse transcriptase
When using a micro centrifuge, what should you check before starting the machine?
A. That the samples are balanced
B. That the PCR tube caps on the samples are slightly open
C. That the machines is set to 64 degrees Celsius
D. That is programed for 35 cycles.
Chapter 26 Solutions
BIOLOGY
Ch. 26.1 - Prob. 1LOCh. 26.1 - Prob. 2LOCh. 26.2 - Prob. 1LOCh. 26.2 - Prob. 2LOCh. 26.3 - Prob. 1LOCh. 26.3 - Describe how viruses can contribute DNA to their...Ch. 26.4 - Describe the differences between acute and...Ch. 26.4 - Prob. 2LOCh. 26.4 - Prob. 3LOCh. 26.5 - Prob. 1LO
Ch. 26 - Prob. 1UCh. 26 - Prob. 2UCh. 26 - Prob. 3UCh. 26 - Prob. 4UCh. 26 - Why is a drug that blocks HIV binding to one of...Ch. 26 - Prob. 6UCh. 26 - Prob. 7UCh. 26 - Prob. 1ACh. 26 - Prob. 2ACh. 26 - Prob. 3ACh. 26 - Prob. 4ACh. 26 - Prob. 5ACh. 26 - Prob. 6ACh. 26 - Prob. 7ACh. 26 - E. coli lysogens derived from infection by phage ...Ch. 26 - Prob. 2SCh. 26 - Prob. 3SCh. 26 - Prob. 4SCh. 26 - Prob. 5S
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- What is used to cut the DNA in panels 1 and 2? a. Restriction enzymes b. DNA polymerases c. DNA ligases d. RNA polymerases e. Reverse transcriptasearrow_forwardWhat technique can be used for studying the gene for cystic fibrosis? a. cellular cloning b. molecular cloning c. reproductive cloningarrow_forwardWhat is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forward
- Choose the combination of answers that most accurately completes the statement. Which gene is incorporated into plasmids to detect recombinant cells? a. restriction endonuclease c. a gene for antibiotic resistance b. virus receptors d. reverse transcriptasearrow_forwardIf you knew the sequence of a gene in one organism, how could you determine if another organism had a similar gene? A. insert the known gene into a vector and use the vector to insert the known gene into the other organism B. treat the genomes of both organisms with the same restriction enzyme and compare the patterns of the bands produced with gel electrophoresis C. create a hybrid of the two organisms by breeding them and check for mutations D. create labeled DNA probes from the known gene and use them to search the genome of the other organismarrow_forwardIf a PCR is started using 10 pieces of template DNA, how many pieces of DNA would there be after 10 cycles? a. About 100 b. About 1000 c. About 10,000 d. About 1010arrow_forward
- DNA fingerprinting analyzes the DNA from individuals on the basis of the occurrence of in their genomes. a. repetitive sequences b. abnormalities in chromosome structure c. specific genes d. viral insertionsarrow_forwardWhich type of cells were used to extract the DNA that was sequenced? a. red blood cells c. white blood cells b. intestinal epithelium d. cheek swab What type of mutation caused Nicholas’s disease? a. frameshift c. nonsense b. missense d. insertionarrow_forwardinvolved in the transfer of multiple drug resistance from one cell to another? * a.Transposition b. Conjugation with a cell with chromosomal drug resistance appears in the genome of a bacteriophage that has infected it. c. Transformation of chromosomal genes d. Conjugation with a cell with a free plasmid carrying drug resistancearrow_forward
- What is the role of a virus in gene therapy?a. It causes the disease that gene therapy is aiming to cure.b. It carries the healthy DNA into the patient’s cells.c. It carries the faulty DNA out of the patient’s cells.d. It reveals which cells carry the DNA causing the disease.arrow_forwardWhich nucleic-acid based technique will detect norovirus? A. Fluorescent In Situ Hybridization (FISH) probes B. PCR with primers specific for the genome of this virus C. Terminal Restriction Fragment Length Polymorphism (T-RFLP) D. reverse transcription PCR with specific primers A patient's blood sample tests positive for the antigen to Vibrio cholerae. Which of the following interpretations is correct? A. This person has cholera. B. This person was exposed to any species of Vibrio. C. This person was exposed to V. cholerae recently. D. This person had cholera at some point in their lifetime.arrow_forwardMatch the process to the step in producing a transgenic plant using the Ti plsmid method. 45. A gene is isolated and the process starts. 46. The recombinant DNA has this so it can be found later. 47. The plasmid enters here. 48. A. tumefaciens infects target organism. 49. The selectable marker allows transgenic plants to survive antibiotic. 50. Transgenic plant cells are isolated. 51. Transgenic plants are prepared for life in the soil. Kanamycin is added to culture. b. The isolated transgenic cells are grown into multicellular organisms. C. Ti plasmid picks up gene of interest. d. Bacterium Agrobacterium tumefaciens takes this up. A selectable marker makes the cell resistant to kanamycin. a. e. f. Antibiotics kill any remaining bacteria. g. The host plant is selected and exposed to the new DNA.arrow_forward
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