Chapter 25, Problem 39CQ

Summary Introduction

To explain: The reason why it is necessary to use only the Klenow fragment, rather than intact E. coli Pol I, in DNA sequencing reactions.

Introduction: A protein fragment that is obtained when the E. coli’s DNA polymerase I is enzymatically cleaved using protease subtilisin enzyme is called as Klenow fragment. DNA sequencing method such as chain termination method is done using Klenow fragment.