BIOLOGY
BIOLOGY
12th Edition
ISBN: 9781260169614
Author: Raven
Publisher: RENT MCG
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Chapter 24, Problem 3IQ
Summary Introduction

To determine: The findings of the gene transcription features in the genomes of apes and humans.

Introduction: DNA replication takes place before the cell division and prepares the cell for mitosis and meiosis. This process is followed by transcription and translation. Genetic testing is one of the most useful tools in diagnostics to identify genetic abnormalities. It helps to detect the presence of any heritable malformation carried through the genetic makeup of the parents to the offspring.

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The figure below shows RNA-Seq data (RED) for the D. melanogaster transformer (tra) gene obtained from both adult female and male fruit flies. The blue lines indicate the tra gene structure, with thicker lines indicating exons, and thin lines introns. The 5' end of the gene is on the left, and the 3' end of the gene is on the right. Based on these data, the most likely conclusion is: Males and females express identical isoforms of tra   Males express more tra RNA than females   The female isoform has fewer amino acids   The female isoform has more amino acids   The male and female isoforms have different 3'UTRs
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By whole-exome sequencing, you have identified an early termination mutation in KLHL4 in a human patient with an undiagnosed blood vessel anomaly. There is almost nothing known about the function of this gene, and no existing animal models! To begin to understand its function, you decide to use the zebrafish model.   You first want to know where in the embryo this gene is expressed. Which technique would you use to identify the cell type that expresses klhl4 mRNA in zebrafish embryos?               You find that this gene is expressed in endothelial cells, which line blood vessels. Intrigued by this finding, you next decide to disrupt the gene in zebrafish using CRISPR/Cas9. The DNA sequence that you want to target is below. What is the sequence of your 20-base guide RNA?     5’ TAGCAATTATGCGCGCTAGCAATTGCGTAGGTCATAATGCAGCTGAC 3’ 3’ ATCGTTAATACGCGCGATCGTTAACGCATCCAGTATTACGTCGACTG 5’         After injecting the gRNA with Cas9, what are potential outcomes? Enter true or false.…
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