Prescott's Microbiology
Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Chapter 23, Problem 5AL
Summary Introduction

A diverse group of bacteria is present in the class of Clostridia. Clostridium is a Gram-positive, fermentative, and obligate anaerobic bacterium. Members belong to this genus have a great useful effect because they form endospores that are resistant to heat. They are responsible for spoilage of food in many cases, even foods that canned. Clostridium contains around a hundred (100) species that include essential pathogen and free-living bacteria.

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In 1971, Dr. Akira Endo, working at the Sankyo company in Tokyo speculated that fungi not only contained antibiotics but also inhibitors of other processes such as cholesterol metabolism. Over a period of several years, he screened more than 6,000 fungal cultures until a positive result emerged. This compound, which was named compactin, came from the blue-green mold Penicillium citrinum Pen-5 that was isolated from a rice sample collected at a grain shop in Kyoto. Based on biochemical assays, compactin is the first known statin. 1. The original kinetics data for compactin inhibition of HMG-CoA reductase enzyme that was published by Dr. Endo in 1976 is shown on the right. a) Determine the Km and Vmax values for enzymatic activity. Do NOT forget the units. Answers can be expressed either as a fraction or in decimals. With No inhibitor: ; Vmax= -60 1/V, (nanomoles/min)-1 0.4 0.35+ 0.3 0.25 0.2 0.15 0.1 0.05 -40 -20 0 + 24 nM compactin + 70 nM compactin None T 20 40 60 1/[HMG-CoA], (mM)-1…

Chapter 23 Solutions

Prescott's Microbiology

Ch. 23.1 - Prob. 1.8CCCh. 23.1 - Retrieve, Infer, Apply List the distinguishing...Ch. 23.1 - Prob. 2.2CCCh. 23.1 - Prob. 2.3CCCh. 23.1 - Retrieve, Infer, Apply Describe three ways in...Ch. 23.1 - Briefly describe the defining properties of genera...Ch. 23.1 - Prob. 2.6CCCh. 23.1 - Examine the ingredients list on a commercial...Ch. 23.2 - What growth or survival advantage might this type...Ch. 23.2 - What is the net yield of ATP for each glucose that...Ch. 23.2 - Prob. 3MICh. 23.2 - Prob. 1CCCh. 23.2 - Prob. 2CCCh. 23.2 - Prob. 3CCCh. 23.2 - Prob. 4CCCh. 23.2 - How are various species of Streptococcus...Ch. 23.2 - Of what practical importance are leuconostocs?...Ch. 23.2 - What is the difference between -hemolysis and...Ch. 23.3 - The presence of an electron transport chain in a...Ch. 23.3 - In addition to its Gram-staining characteristics,...Ch. 23.3 - What are the two sources of electrons for the H....Ch. 23.4 - Prob. 1CCCh. 23.4 - Suggest why C. tetani uses a sodium motive force...Ch. 23.4 - Prob. 3CCCh. 23.4 - What kind of genetic evidence, in addition to 16S...Ch. 23 - Prob. 1RCCh. 23 - Prob. 2RCCh. 23 - Prob. 3RCCh. 23 - Prob. 4RCCh. 23 - Prob. 5RCCh. 23 - Prob. 6RCCh. 23 - Prob. 7RCCh. 23 - Prob. 8RCCh. 23 - Prob. 9RCCh. 23 - Prob. 10RCCh. 23 - Even though actinobacteria are high G + C...Ch. 23 - Given that mycolic acids are essential for...Ch. 23 - Prob. 3ALCh. 23 - Prob. 4ALCh. 23 - Prob. 5ALCh. 23 - What physiological properties might account for...Ch. 23 - S. aureus strains that are resistant to...Ch. 23 - Prob. 8AL
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