Why is the wavelength of the light that is released in spectrophotometric analysis longer than the wavelength of the light that is used to activate a fluorescent molecule?

Which pigment spots (or bands) produce fluorescence under long-wave ultraviolet light?

What type of probes must be used for super-resolutionmicroscopy and why?

In light microscopy, when magnification is increased, the lens focuses closer to the sample.  This makes the user more likely to ram the sample into the lens.  Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?

1) What happens to the cells when you heat-fix them, and what are the two goals or purposes of heat-fixing cells prior to staining them? 2) How do direct stains work, that is, how or why do direct stains stain? 3) Why do cells appear to be white or colorless when you use indirect stains?

What is the advantage of phase-contrast microscopyover bright-field microscopy?

What is phase contrast? Give examples of phase contrast in optical microscopy.

In what ways are dark field microscopy and negative staining alike?

Briefly explain the difference between Wright Staining and H&E Staining (why use one over the other)

You are asked to design an assay to measure the concentration of WonderDrug in various different pharmaceutical preparations using either its UV-visible absorption or its fluorescence emission. Explain when a UV-visible absorption based assay would be the more appropriate and when a fluorescence based assay would be more appropriate.