Concept explainers
(a)
Interpretation:
The reason the proteins are not visible in the lanes without amylase treatment should be discussed.
Concept introduction:
Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.
SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.
(b)
Interpretation:
The effect of treating the sample with a-amylase should be explained.
Concept introduction:
Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.
SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.
(c)
Interpretation:
The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.
Concept introduction:
Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.
SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.
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Biochemistry
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