(a)
Interpretation:
The results obtained with the mutant should be explained.
Concept introduction:
Glycogen phosphorylase catalyzes the cleavage of the terminal alpha-1,4-glycosidic bond of glycogen
(b)
Interpretation:
The effect of substituting aspartic acid for serine should be predicted.
Concept introduction:
Glycogen phosphorylase catalyzes the cleavage of the terminal alpha-1,4-glycosidic bond of glycogen polymer releasing glucose-1-phosphate. The most important regulatory site in glycogen phosphorylase is Serine 14 residue. Phosphorylation of this ser-14 residue activate phosphorylase b form to phosphorylase a form.
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Biochemistry
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