Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 19.3, Problem 3R
Summary Introduction
To review:
The condition where the Nanos were inserted and overexpressed at the anterior end of the Drosophila embryo.
Introduction:
There are two maternal effect genes present in the Drosophila, which are known as the bicoid and the Nanos. They are responsible for the determination of the anterior–posterior axis of the embryonic egg.
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The following diagram illustrates four genes from the genome of a certain insect. Different binding
sites are labeled in the enhancer region of each gene.
enhancer
promoter
ABC D E
Gene 1
A
D
Gene 2
АС
DE
Gene 3
ABD
Gene 4
In order for a specific gene to be expressed in the insect's cells, all of the gene's binding sites must
be bound by transcriptional activators.
• The insect's brain cells contain activators that bind to sites A, C, D, and E
• The insect's salivary gland cells contain activators that bind to sites A, B, and D
Which of the following is the most likely pattern of gene expression for the insect described above?
Gene 2 will be expressed in both brain and salivary gland cells
Both gene 1 and gene 4 will be expressed in salivary glands, but neither will be expressed in brain cells
Gene 1 will be expressed in both brain and salivary glands
Both gene 2 and gene 3 will be expressed in brain cells, but neither will be expressed in salivary gland cells
What would be the most likely effect of inhibiting the translation of hunchback mRNA throughout a Drosophila embryo?
What would be the most likely result of injecting bicoid mRNA into the posterior end of a Drosophila embryo and inhibiting the translation of nanos mRNA?
Chapter 19 Solutions
Life: The Science of Biology
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- Please search and list a number of promoter sequences in DNA vectors that are compatible with mammalian cell expression system.arrow_forwardQ1: As illustrated here, at what control point is transcription regulated? Q2: What is a possible advantage of regulating gene expression before transcription, versus after? Q3: If you wanted to up-regulate production of the hemagglutinin protein in a tobacco plant carrying the hemagglutinin gene, at which control point(s) would that be possible? Justify your reasoning.arrow_forwardA Drosophila egg that is bed lbcd may develop normally, but the adult fruit fly will not be able to produce viable offspring. How is this possible? The mother can provide the bcd mRNA required for normal development, but the adult bcd /bcd female fly cannot make its own bcd mRNA. The Hox genes in the egg play a redundant role to bcd during development in a bcd / bcd egg, but reproduction requires bcd. Drosophila sperm contains the functional bcd gene product required for normal development, but the adult sex cells will not be able to produce the functional product. The nanos gene product functionally compensates for lack of bcd expression in the developing fly but not in its offspring.arrow_forward
- Based on the given scenario, tell whether structural genes are expressed (YES) or not expressed (NO) 1. LacY is deleted (YES/NO) 2. LacI is deleted (YES/NO) 3. TrpR is deleted (YES/NO) 4. Promoter sequence is deleted (YES/NO) 5. lactose is present in the environment; glucose is absent (YES/NO) 6. tryptophan is absent in the environment (YES/NO) 7. operator is deleted (YES/NO)arrow_forwardSuppose you want to determine whether a particulargene X is important for specification of the pharynx,but mutations in this same gene disrupt embryonicdevelopment well before pharyngeal structures appear.How could you use myo-2::GFP, the myo-2 promoter,the DNA sequence of gene X, and your knowledge ofRNA interference (RNAi) to generate worms that lackgene X expression in the pharynx but express gene Xin all other tissues in which it is expressed in wildtype C. elegans?arrow_forwardSuppose expression of gene A is limited to the middle part of the early mouse embryo. Expression of gene B is located on the posterior and anterior ends of the early mouse embryo, but not in the middle. When gene B is mutated, expression of gene A is distributed over the whole embryo. What is a likely explanation for this data? a) Gene A acts as an activator of gene B. Ob) Gene A acts as a repressor of gene B. O c) Gene B acts as an activator of gene A. () d) Gene B acts as a repressor of gene A.arrow_forward
- A translocation mutation results in a silencer being inserted just upstream of the promoter of a gene (Gene X). How would you predict the expression of gene X be affected by this mutation? Gene X will not be expressed in any cell that has this mutation Gene X will not be expressed in any cell that has this mutation and the appropriate repressor protein Gene X will be expressed in any cell that has this mutation Gene X will be expressed in any cell that has this mutation and the appropriate repressor proteinarrow_forwardSuggest a direct experiment to prove that p53 binding at gene promoters affects the level of gene expression of the corresponding genes.arrow_forwardIn the regulatory switch experiment, what do you predict is a likely outcome in mice with the bat-mouse construct? Select one 1.) change in the nucleotide sequence of the Prx1 transcribed region 2.) change in the amount of Prx1 mRNA produced 3.)change in the amino acid sequence of the PRX1 protein 4.)change in the nucleotide sequence of the Prx1 mRNA producedarrow_forward
- During certain stages of development, the requirement forcertain gene products may require gene amplification. Whatpurpose does gene amplification serve?arrow_forwardE27. A cloned gene fragment contains a regulatory element that is recog- nized by a regulatory transcription factor. Previous experiments have shown that the presence of a hormone results in transcriptional acti- vation by this transcription factor. To study this effect, you conduct a electrophoretic mobility shift assay and obtain the following results: Tube: 1 2 3 Transcription factor: Hormone: Explain the action of the hormone.arrow_forwardExpression of recombinant proteins in yeast is an important tool for biotechnology companies that produce new drugs for human use. In an attempt to get a new gene X expressed in yeast, a researcher has integrated gene X into the yeast genome near a telomere. Will this strategy result in good expression of gene X? Why or why not? please try to explain a bit elaborately.arrow_forward
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