what do you predict is a likely outcome in mice with the bat-mouse construct? Select one 1.) change in the nucleotide sequence of the Prx1 transcribed region 2.) change in the amount of Prx1 mRNA produced 3.)change in the amino acid sequence of the PRX1

A translocation mutation results in a silencer being inserted just upstream of the promoter of a gene (Gene X).  How would you predict the expression of gene X be affected by this mutation? Gene X will not be expressed in any cell that has this mutation Gene X will not be expressed in any cell that has this mutation and the appropriate repressor protein Gene X will be expressed in any cell that has this mutation Gene X will be expressed in any cell that has this mutation and the appropriate repressor protein

Based on Pruisner (2013), match each of the following terms with its appropriate definition or description.                                                                         PRNP gene                                                                        CPEB (cytoplasmic polyadenylation element binding) protein                                                                        NFT (neurofibrillary tangle)                                                                        A673T-mutated AAP                                                                        MAPT gene A. a "nonpathogenic prion" B. encodes PrPC (the normal cellular form of prion protein) C. encodes tau protein;  mutations in this gene underlie many familial FTDs (frontotemporal dementias) D. tau-rich fibril associated with many neurodegenerative diseases E. a missense mutation in β-secretase which appears to protect against late-onset…

This chapter describes different types of TEs, including insertionelements, simple transposons, LTR retrotransposons, and non-LTRretrotransposons. Which of these four types of TEs have the followingfeatures?A. Require reverse transcriptase to transposeB. Require transposase to transposeC. Are flanked by direct repeatsD. Have inverted repeats

which laboratory method can be used to quantify levels of mRNAs expressed in samples of two different types of stem cells?

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Your identify a number of mutants and start to characterize the problems in their mismatch repair mechanisms.  Based on the following initial results of your experiments, predict which mismatch repair protein(s) is mutated in each mutant.  (Your experiments tells you what mismatches are being left unfixed.) Mutant 1:  Still contains mismatches where G binds to T and T          binds to G Mutant 2:  Roughly one half of all mismatches remain. Mutant 3:  All mutations remain, but you find that no mismatch             repair proteins are non-functional. Mutant 4:  All mutations remain, and you find increased MutL-MutS          complexes bound to the DNA.

The protein Menchie is conserved from cats to humans. The region upstream of the menchie gene has three conserved sequences: region A, B, and C. Mutations were made in this upstream region by removing these regions or changing the orientation of these regions. Additionally, the coding sequence for Menchie was replaced with the coding sequence for green fluorescent protein (GFP; gene = gfp). GFP fluorescence was measured for each of the indicated constructs and the results are summarized. The arrow indicates the direction of transcription and where transcription starts (the +1 site). c. Which region (A, B, or C) contains an enhancer sequence? Briefly explain your answer, using at least two lines of evidence from the results shown; please use the #s to indicate the line of evidence you are describing. d. Which region (A, B, or C) contains a silencer sequence? Briefly explain your answer, using at least two lines of evidence from the results shown; please use the #s to indicate the line…

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Wnt4 is expressed in a variety of embryonic tissues. You decide to study this gene during mouse development Part 1 In the mouse embryo at gastrulation, are Wnts concentrated at the anterior or posterior region? Part 2 You decide to conduct a Wnt4 immunoblot (Western blot), using tissues from mouse embryos. What key reagent do you need to carry out this method? Part 3 Your immunoblot shows that Wnt4 is expressed in the embryonic ovary. What also needs to be expressed in the ovary for Wnt4 to have an effect there? Part 4 Outline in three dot points how Wnt4 can induce gene expression in neighbouring cells. Part 5 a. What method you would use to show that Wnt4 gene is required for ovary development in the mouse? b. In three dot points, outline how this method is carried out.