You isolate bacteria from several different pools at the recreation center. Curious about which bacteria likely have a common ancestor with your reference pool (the Buffalo-pool), you BLAST the 16S ribosomal gene from the Buffalo pool bacteria against the 16S ribosomal genes from the other pools. The results are shown below. Which of the bacteria likely have a common ancestor with the Buffalo-pool bacteria? Use e as a cutoff for homology. Organism Dive-well bacteria Competition-pool bacterial Practice-pool bacteria Puddle bacteria U Puddle bacteria Practice-pool bacteria Competition-pool bacteria Dive-well bacteria E-value 9e-175 -300 8e 4e-2 4

Bacteria and other microbes can be used to "clean up" an oil spill by breaking down oil into carbon dioxide and water. Two samples isolated from the Deepwater Horizon leak in the Gulf of Mexico were labeled A and B. The DNA of each was isolated and the percent thymine measured in each sample. Sample A contains 19.3% thymine and sample B contains 27.9% thymine. Assume the organisms contain normal double-stranded DNA and predict the composition of the other bases. percent adenine in sample B: percent guanine in sample B: percent cytosine in sample B: Both samples are then denatured to remove the secondary structure. Which will have the higher temperature to denature? A B % A %G %C

6) You sequence the genomes of 100 strains of bacteria and want to select a bar coding gene to assess how closely they are related. Name three criteria you will use to select a gene to analyze.

What are the factors that you and your team will consider to ensure that the "oil- eating" bacteria you chose will multiply, grow, and thrive efficiently in the Kalba sea area? Provide at least 3 factors using complete sentences.. A genetic technology firm wants to collaborate with your team by offering their latest genetic technology advancement, which consists of using eukaryotic cells that have the ability to take up and digest oil components. Describe the advantages and disadvantages of using eukaryotic cells for cleaning oil spills. Would you consider this option for the treatment of the infected site? Explain why or why not using complete sentences. In your answer, compare prokaryotes versus eukaryotes in terms of the organelles that capture and release energy and their modes of reproduction.

After you design the PCR primers, you run the PCR on fluid from the patient’s nasal swab. Next, you need to evaluate the results. PCR makes billions of copies of just one sequence in a sample. Since you know the sequence, you also know the length (number of nucleotides) of the region you copied. There are about 130 nucleotides in the N gene fragment. This is typically stated as 130 base pairs. How can you visualize DNA and estimate its size? Load the DNA sample into an agarose gel (similar in consistency to jello) and apply an electric current to the gel. The DNA is charged and will move through the gel. The longer the DNA fragment, the more slowly it moves. The DNA is visualized by adding a fluorescent dye to your sample that sticks to DNA. When you look at the gel under UV light, the DNA should glow. What is the charge of a DNA molecule? Based on #1, would you expect DNA to be drawn to the (+) or (-) electrode of the gel electrophoresis chamber?

Why bacteriophage is called t4?

With the technique of interrupted mating four Hfr strains were tested for the sequence in which they transmitted a number of different genes to an F strain. Each Hfr strain was found to transmit its genes is a unique sequence, as shown in the accompanying table. What is the gene sequence in the original strain from which these Hfr strains were derived?

The unicellular, rod-shaped bacterium E. coli is~2 μm long and 0.8 μm wide, and has a genomeconsisting of a single 4.6 Mb circular DNA molecule. The unicellular archaean Methanosarcinaacetivorans is spherical (coccus-shaped) with adiameter of 3 μm and has a 5.7 Mb circular genome.The unicellular eukaryote Saccharomyces cerevisiaeis roughly spherical, with a diameter of 5–10 μm.It has a haploid genome of 12 Mb divided among16 linear chromosomes. Given these descriptions,how could you determine whether a new, uncharacterized microorganism was a bacterium, an archaean,or a eukaryote?

A mutation occurs in a single bacterium that causes it to produce a new enzyme. This enzyme helps the bacterium process nutrients more efficiently, increasing the bacterium's odds of survival. Every 30 minutes, the bacteria in the colony divide, passing their genes on to their offspring. Some bacteria survive, while others do not. Because resources are limited, the colony has a steady population of 114 bacteria. The bacterial colony is shown below at three different time intervals.   The picture would be here:     Assume that the survival trend shown in the diagram continues. At 3.0 hours, the mutant bacteria will make up _____ percent of the entire colony.

Describe briefly the perks, disadvantages and use of 16s rRNA genes in taxonomic level of classification of bacteria.  Cite the claims to be discussed, only here: https://journals.asm.org/doi/full/10.1128/CMR.17.4.840-862.2004 Create a review format of the task.