Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Question
Chapter 19, Problem 16P
Interpretation Introduction
Interpretation:
The various sources of protons which participate in the generation of a proton gradient in chloroplastsare to be stated.
Concept introduction:
Photosynthesis is a reaction in which carbon dioxide and water combine together in the presence of sunlight to form glucose and oxygen. The organelle of plants which helps in the conduction of photosynthesis at where the chlorophyll traps the energy obtained from sunlight is known as chloroplasts.
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Chemiosmosis. ATP synthesis in chloroplasts is usually light dependent; it does not happen in the dark.
When researchers shifted isolated chloroplasts from a low pH solution to a more alkaline (higher pH) solution, ATP synthesis occurred even in the absence of light! This was an experiment used to support the chemiosmosis mechanism of ATP formation in chloroplasts. Why?
What would happen if the isolated chloroplasts were shifted to a lower pH solution?
What would be the results if the above experiments were performed in the presence of light?
If these experiments were performed in mitochondria instead of chloroplasts, what results would we expect?
Fo-F1 ATPase. The energy for ATP synthesis from ADP and Pi is provided by the downhill transport of protons through the rotary FoF1 ATP synthase . The enzyme has 3 alpha-beta and 12 ‘c’ subunits. The mitochondrion maintains change in membrane potential=180 mV (negative inside), pHin = 8, pHout=7, [Pi] = 3 mM and ADP is present as well.
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Chapter 19 Solutions
Biochemistry
Ch. 19 - Prob. 1PCh. 19 - Prob. 2PCh. 19 - Prob. 3PCh. 19 - Prob. 4PCh. 19 - Prob. 5PCh. 19 - Prob. 6PCh. 19 - Prob. 7PCh. 19 - Prob. 8PCh. 19 - Prob. 9PCh. 19 - Prob. 10P
Ch. 19 - Prob. 11PCh. 19 - Prob. 12PCh. 19 - Prob. 13PCh. 19 - Prob. 14PCh. 19 - Prob. 15PCh. 19 - Prob. 16PCh. 19 - Prob. 17PCh. 19 - Prob. 18PCh. 19 - Prob. 19PCh. 19 - Prob. 20PCh. 19 - Prob. 21PCh. 19 - Prob. 22PCh. 19 - Prob. 23PCh. 19 - Prob. 24PCh. 19 - Prob. 25PCh. 19 - Prob. 26PCh. 19 - Prob. 27PCh. 19 - Prob. 28PCh. 19 - Prob. 29PCh. 19 - Prob. 30PCh. 19 - Prob. 31P
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- photosynthesis. Historical Discovery Process The chemical changes that occur during photosynthesis have been investigated for the past 300 years. The general highlights of these discoveries help us to understand the basic process of photosynthesis. Three centuries ago people wondered where plants came from. They knew that plants grew out of the ground, but how that happened was a complete mystery to them. The first step in answering the question was to plant a small tree in a large pot supported off the ground. They did this so that the soil of the container was separated from the soil of the earth. Only the dirt in the pot was available to the plant. The people cared for the plant during one year. At the end of the year the small tree had gained 100 kg. (The actual data from this experiment have been changed to simplify the discussion.) 1. What do you think the experimenters considered as two possible sources for the substances (matter) that became incorporated into new tree growth?…arrow_forwardChemical energy. The diagram below shows a metabolic pathway that occurs in chloroplasts during photosynthesis. ribulose bisphosphate (RuBP) ADP + P₁ carbon dioxide (CO₂) 1 X HINEW ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCo) glucose triose phosphate (TP) State two features of metabolic pathways? two key features of metabolic pathways glycerate-3-phosphate (GP) are their -X ADP + P₁ interconnectednearrow_forwardNeed help ASAP. Describe the steps by which the F0 portion of the ATP synthase harnesses the proton-motive force to help synthesize ATP. What would you expect to observe if the proton gradient were reversed? Explain your answer.arrow_forward
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