Genetic Analysis: An Integrated Approach (3rd Edition)
Genetic Analysis: An Integrated Approach (3rd Edition)
3rd Edition
ISBN: 9780134605173
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 18, Problem 9P
Summary Introduction

To analyze:

The activity of RNA polymerase II is inhibited by Actinomycin D. When actinomycin D is present, early development in many vertebrate species, for example frogs, can begin after the formation of a blastula (hollow ball of cells that forms after early cleavage divisions), but development stops before gastrulation. Question asked to discuss the activity of maternal gene in contrast to activity of zygotic gene in early frog development.

Introduction:

Drosophila is the model organism of study of development. It is a well studied genome sequence. Multiple genes are involved in the development of drosophila. The gene that directs the development and fate of an organism are known as developmental genes. Bicoid and Nanos are maternal effect genes; the egg receives these from the mother. Bicoid and Nanos play an important role in creating the anterior to posterior axis. The genes are activated in the fertilized egg (Zygote). In contrast to maternal effect genes, these genes are transcribed by zygotic DNA.

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Chapter 21 describes two blotting methods (i.e., Northern blotting and Western blotting) used to detect gene products. Northern blotting detects RNA and Western blotting detects proteins. Suppose that a female fruit fly is heterozygous for a maternal effect gene, which we will call gene B. The female is Bb. The normal allele, B, encodes a functional mRNA that is 550 nucleotides long. A recessive allele, b, encodes a shorter mRNA that is 375 nucleotides long. (Allele b is due to a deletion within this gene.) How could you use one or more of these techniques to show that nurse cells transfer gene products from gene B to developing oocytes? You may assume that you can dissect the ovaries of fruit flies and isolate oocytes separately from nurse cells. In your answer, describe your expected results.
Using a transgenic technique, propose an experiment to determine whether Cdx2 is sufficient for trophoblast development in the mouse embryo. Describe two results that you would expect to observe at the blastocyst stage if Cdx2 is indeed sufficient for trophoblast development. Be as specific as possible regarding the transgene that you propose for this experiment (including what gene's enhancer you would use in the transgene). Note: you do not need to explain the details of how a transgenic mouse is made. Describe the experiment in steps (Step 1: ..., Step 2: ... etc) and please keep your answer to under 150 words.   tips:  DONT talk about stop cassetes/memory cassetes, focus on transgenes Paper called "Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst" gave lots of results that you might see,, 6 diff ways that cdx2 is required for trophoblasts need specific gene enhancer (dont just say "expressed enhancer in genital…
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Genetic Analysis: An Integrated Approach (3rd Edition)

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