Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 17, Problem 2VC
From this chapter, figure 17.14. Can you explain why one single bottle of secondary antibody can serve as the indicator antibody for any ELISA reaction?
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Students have asked these similar questions
Conjugated enzymes are a key component of ELISA. To what are these enzymes conjugated, and why is that important?
To the antigen so the enzymes are only present when the molecule of interest is also present
To the plastic of the reaction well so the enzymes can concentrate the molecule of interest
To the capture antibody so the enzymes are only present when the molecule of interest is also present
To the detection antibody so the enzymes are only present when the molecule of interest is also present
What are the ordered steps of
an ELISA protocol?
A. Add primary antibody->wash->
Bind sample to a surface ->Add substrate
->Add secondary antibody-enzyme
conjugate ->wash
B. Bind sample to support -> Add
substrate -> Add primary antibody ->
wash -> Add secondary antibody-enzyme
conjugate -> wash
C. Bind sample to a surface -> Add
primary antibody -> wash -> Add
secondary antibody-enzyme conjugate ->
wash -> Add substrate
D. Add secondary antibody-enzyme
conjugate -> wash -> Add primary
antibody -> wash -> Add substrate -> Bind
sample to surface
Many ELISAs that are used to identify the presence of antibody to viruses have a 3rd control (along with negative and positive). In between the rows of wells coated with antigen, are rows of cells coated with tissue culture used to grow the virus. In other words, patient serum is added to wells that contain antigen along with wells that contain tissue culture. Why do you think this might be necessary?
Chapter 17 Solutions
Microbiology: A Systems Approach
Ch. 17.1 - Prob. 1AYPCh. 17.1 - Prob. 2AYPCh. 17.1 - Prob. 3AYPCh. 17.1 - Prob. 4AYPCh. 17.2 - Summarize the most common methods used for direct...Ch. 17.2 - Prob. 6AYPCh. 17.2 - Prob. 7AYPCh. 17.3 - Prob. 8AYPCh. 17.3 - Differentiate between sensitivity and specificity.Ch. 17.3 - Explain how fluorescent antibodies can be used in...
Ch. 17.3 - Prob. 11AYPCh. 17.3 - Prob. 12AYPCh. 17.3 - Prob. 13AYPCh. 17.3 - Explain the difference between a direct and an...Ch. 17.4 - Prob. 15AYPCh. 17.4 - Prob. 16AYPCh. 17.4 - Prob. 17AYPCh. 17.5 - Prob. 18AYPCh. 17.5 - List the major advantages of microarray methods of...Ch. 17.5 - Summarize the benefits of using whole-genome...Ch. 17 - Prob. 1MCQCh. 17 - Prob. 2MCQCh. 17 - Prob. 3MCQCh. 17 - Prob. 4MCQCh. 17 - Prob. 5MCQCh. 17 - Prob. 6MCQCh. 17 - Prob. 7MCQCh. 17 - Prob. 8MCQCh. 17 - The Western blot test can be used to identify a....Ch. 17 - Prob. 10MCQCh. 17 - True-False Questions. If the statement is true,...Ch. 17 - Prob. 12TFCh. 17 - Prob. 13TFCh. 17 - Prob. 14TFCh. 17 - Prob. 15TFCh. 17 - Explain why specimens should be taken aseptically,...Ch. 17 - Prob. 2CTQCh. 17 - Explain which type of ELISA can be used to...Ch. 17 - Prob. 4CTQCh. 17 - Prob. 5CTQCh. 17 - Prob. 1VCCh. 17 - From this chapter, figure 17.14. Can you explain...Ch. 17 - Prob. 1CM
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- If you were using the ELISA to look for the presence of antibodies and the sample gave a negative result, does this mean that there were no antibodies present? Explain.arrow_forwardCounterstaining with Hematoxylin and Eosin is an important step in which of the following techniques? ELISA Gel Electrophoresis IHC Western blottingarrow_forwardThe secondary antibodies used in the ELISA experiment are conjugated (attached) to what enzyme? PRP HRP Rh АВОarrow_forward
- Predict the outcome of an error in ELISA protocol consisting of adding the antibodies in the wrong order. * i.e., the secondary antibody is added 1st; the primary is added 2nd Your answerarrow_forwardIs rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid antibody testing? Describe how antibodies are used to detect the presence of a specific disease.arrow_forwardYou are interested in performing indirect immunofluorescence light microscopy to observe the localization of the catalase enzyme in the cultured HeLa cells, obtained historically from the cervical tumor of Henrietta Lacks. You were going through the lab stock and found a few primary and secondary antibodies. Which of the following secondary antibody can you use in your experiments? O All of the mentioned antibodies can be used in the experiment Goat anti-human antibody conjugated to 10 nm gold Goat-anti-human catalase conjugated to 10 nm gold O Human anti-catalase antibody conjugated to fluorescent rhodamine Goat anti-human antibody conjugated to fluorescent rhodaminearrow_forward
- Anita suspects she might have had Covid-19 about four months ago. Currently, she has no symptoms. What tests should be used to find out if that indeed was the case? cell culture Indirect ELISA Direct ELISA real-time qPCRarrow_forwardWrite down basic principle of ELISA and its main steps? Write names of different types of ELISA. (Subject: Biotechnology)arrow_forwardDifference between Antibody ELISA and Antigen ELISAarrow_forward
- You have 4 ml of an antigen solution, how would you prepare a 3-fold dilution series such that you will have at least 2 ml of each dilution? Please draw a picture and explain, I don't understand what it means by 3-fold. Please explain, no copy paste asaparrow_forwardIn an ELISA, which of the following caused a blue color change? O The primary antibody O The secondary antibody O The enzyme/substrate reaction O hCGarrow_forwardYou performed an Elisa test in the lab to detect antibody against HIV in the patient's serum. The test was found to be 96% sensitive. What does that mean? A western blot was used on the same sample to confirm, and the test was found to be 99% specific. What does that mean?arrow_forward
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