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- Anita suspects she might have had Covid-19 about four months ago. Currently, she has no symptoms. What tests should be used to find out if that indeed was the case? cell culture Indirect ELISA Direct ELISA real-time qPCRin an indirect elisa procedure what enzyme is used?What other test can be used to confirm the results obtained in this ELISA test? Name and very briefly describe one other test. Give the reason for your choice.
- What is the role of horseradish peroxidase conjugate in the ELISA experiment? Antigen Primary antibody Second antibody SubstrateWhat is a secondary antibody in an ELISA test?a) did the positive and negative controls work correctly in this ELISA? Explain. B) in the sample wells( wells3-7) some are darker blue than others. Explain what happened here and how you'd interpret the results?
- What other test can be used to confirm the results obtained for a TEV infected tomato in an ELISA test? Name and very briefly describe one other test. Give the reason for your choice.Why does the antibody titer determination use twofold dilutions ofthe antiserum rather than 10-fold dilutions?What are the differences between “O” and “H” antigen? Differentiate Widal from Weil-Felix test. The strains of Proteus vulgaris are used in Weil-felix reaction. Explain why.
- What is the difference between an indirect ELISA and a sandwich ELISA? Is rapid antibody testing a form of ELISA?which of the following are necessary to run a valid elisa test?ELISA tests usually use a primary and secondary antibody. Why? What are the necessary controls one would need to perform to ensure that the antibody specificities are valid (i.e., no false-positive or falsenegative reactions)?