Microbiology: A Systems Approach
Microbiology: A Systems Approach
5th Edition
ISBN: 9781259706615
Author: Marjorie Kelly Cowan Professor
Publisher: McGraw-Hill Education
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Chapter 17, Problem 12TF
Summary Introduction

Introduction:

Nucleic acid hybridization is a technique in which a single stranded DNA or RNA is used to bind to its complementary DNA or RNA. A double-stranded DNA molecule is generally stable, but it is denatured at high temperatures.

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Can you please check my answer and make sure it is correct.    Question: How can DNA evidence be used to convict or exonerate a defendant? Why is DNA evidence so powerful?    Answer: DNA evidence can be used to perform DNA profiling to determine the genotype of the specific DNA sample. With just a small amount of DNA, PCR can produce billions of copies of that specific segment. The segments that are used are from non-coding regions that contain STR’s or short tandem repeats. These very short DNA sequences are repeated and are specific to individuals because we inherit them from our mother and father. Gel electrophoresis separates the PCR products based on their size and each band is compared to the allele ladder. This process helps to identify the alleles present in the original samples. DNA profiling is performed at many loci to be able to tell the genetic difference between different individuals with a lot of certainty. The DNA from the different suspects is compared to the allele…
Directions: The following story is about a crime solved through biotechnological techniques. The techniques used are DNA fingerprinting and polymerase chain reaction (PCR). DNA fingerprinting is a technique in which an individual’s DNA is analyzed to reveal the pattern of particular short nucleotide sequences. This pattern is claimed to be unique to the individual concerned and can thus be used for identification purposes. Polymerase chain reaction, on the other hand, is a technique used to replicate a DNA fragment so as to produce many copies of a particular DNA sequence. Why do you think there’s a need to generate several copies of the DNA? You will learn the answers to this as you go through the story. Read the story carefully and answer the questions that follow.   Questions: 1. How did the investigators conclude that the suspect was indeed the murderer?  2. What if a strand of hair was found in the truck bed instead of a seed pod, do you think it will still lead to the…
Directions: The following story is about a crime solved through biotechnological techniques. The techniques used are DNA fingerprinting and polymerase chain reaction (PCR). DNA fingerprinting is a technique in which an individual’s DNA is analyzed to reveal the pattern of particular short nucleotide sequences. This pattern is claimed to be unique to the individual concerned and can thus be used for identification purposes. Polymerase chain reaction, on the other hand, is a technique used to replicate a DNA fragment so as to produce many copies of a particular DNA sequence. Why do you think there’s a need to generate several copies of the DNA? You will learn the answers to this as you go through the story. Read the story carefully and answer the questions that follow.
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