Life: The Science of Biology
11th Edition
ISBN: 9781319010164
Author: David E. Sadava, David M. Hillis, H. Craig Heller, Sally D. Hacker
Publisher: W. H. Freeman
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Chapter 15.4, Problem 2R
Summary Introduction
To review:
The process by which allele-specific oligonucleotide hybridization can be used to detect sickle-cell anemia.
Introduction:
Sickle-cell anemia is genetic disorder in which abnormal rigid, sickle-shaped hemoglobin is produced in red blood cell (RBC). Hemoglobin is responsible for caring and transmitting oxygen to the several parts of the body. Rigid sickle-shaped hemoglobin fails to transmit proper oxygen to the body parts. These result in chronic pain, swelling of hand and feet, stroke, etc.
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DNA obtalned from the indicated donor strains is used to TRANSFORM the indicated recipient strains. The resulting progeny are plated on minimal medium so that only wild-
type recombinants are scored. The number of wild-types for each cross is given in the chart below. What is the order of the genes?
Donor
Recipient
wid type colonies
a-b- c+
a+ b+ c-
273
a- b+ c-
a+ b-c+
462
a- b+ C+
a+ b-c-
2
a b- C+
a- b+ c+
O 4-b-c
a-o-b
b-a-c
cannot be determined
Question 18 of 26
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Life: The Science of Biology
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- DNA obtained from the indicated donor strains is used to TRANSFORM the indicated recipient strains. The resulting progeny are plated on minimal medium so that only wild-type recombinants are scored. The number of wild-types for each cross is given in the chart below. donor donor arg+ val- ser- val+ ser- arg- val- ser+ val- ser+ arg+ val- ser- O valine recipient recipient none of these. O arginine arg- val+ ser+ arg+ val- ser+ Which locus is closest to serine? arg+ val+ ser- wild-types wild-types 70 98 0 2 arg- arg-arrow_forwardISSR is generally a dominant STS DNA marker. Nonetheless, with validated experimental evidence (e.g. laboratory and population genetics data), the marker can be used in codominance marker genotyping. Briefly explain each case below: a) Codominant marker targets specific locus and reveals allelic variations in that locus among DNA samples. b) Dominant marker: primers can complement other repeat sequences or in multiple loci thereby non-specificity in sampled genomes.arrow_forwardDetermine the possibilities to carry out a complementation test on two dominant mutations.arrow_forward
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