Biology (MindTap Course List)
11th Edition
ISBN: 9781337392938
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Chapter 15.2, Problem 2C
Summary Introduction
To explain: How a gene might be deleted in a zygote using CRISPR/Cas9 editing.
Concept introduction: CRISPR or “clustered regularly interspaced short palindromic repeats” are short viral origin DNA repeats that are found in bacteria. Cas is CRISPR-associated endonucleases enzyme that excises the DNA. Thus, CRISPR-Cas9 recognizes the invading virus and cleaves it.
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Describe how a point mutation in a eukaryotic gene could be corrected using the CRISPR/Cas9 editing system.
Describe how the CRISPR-Cas system is used to modify genomes
Please place the stages of a CRISPR-cas9 gene editing workflow in the correct order below. Note, there is one incorrect and state why?
Sequence
Select for correctly gene edited cells (using antibiotic resistance and/or colour production for example)
Clonal isolation
Synthesise DNA insert oligonucleotides
Clonal characterisation (analysis of phenotype)
Add green fluorescent protein gene sequence into plasmid to aid selection of correctly transfected cells.
Transfect cells
Clone into CRISPR-cas9 expression vector
Purify plasmids
Design a set of targeting sequences using algorithms.
Chapter 15 Solutions
Biology (MindTap Course List)
Ch. 15.1 - Prob. 1LOCh. 15.1 - Explain how gel electrophoresis is used to...Ch. 15.1 - Describe how PCR is used to amplify a specific...Ch. 15.1 - Compare the possible differences between a...Ch. 15.1 - Prob. 1CCh. 15.1 - Different forms of a protein are produced in the...Ch. 15.1 - What advantages does the PCR method have over gene...Ch. 15.2 - Describe the features of a typical CRISPR locus in...Ch. 15.2 - Explain the function of CRISPR in bacterial cells.Ch. 15.2 - Compare CRISPR-based endonucleases with...
Ch. 15.2 - Prob. 8LOCh. 15.2 - Prob. 1CCh. 15.2 - Prob. 2CCh. 15.2 - Prob. 3CCh. 15.3 - Prob. 9LOCh. 15.3 - Prob. 10LOCh. 15.3 - Discuss how qPCR, DNA microarrays (DNA chips), and...Ch. 15.3 - Explain how you would compare the expression of a...Ch. 15.3 - Prob. 2CCh. 15.4 - Describe how genome-wide association studies have...Ch. 15.4 - Explain how targeted gene silencing and knockout...Ch. 15.4 - Prob. 1CCh. 15.5 - Describe at least one important application of DNA...Ch. 15.5 - Prob. 1CCh. 15.5 - What are short tandem repeats (STRs), and why are...Ch. 15.5 - Why do gene targeting and mutagenesis screening in...Ch. 15.6 - Prob. 15LOCh. 15.6 - Prob. 16LOCh. 15.6 - Prob. 1CCh. 15.6 - Prob. 2CCh. 15.7 - Describe at least two safety issue associated with...Ch. 15.7 - What are some of the environment concerns...Ch. 15 - A plasmid (a) can be used as a DNA vector (b) is a...Ch. 15 - DNA molecules with complementary sticky ends...Ch. 15 - Prob. 3TYUCh. 15 - Which technique rapidly replicated specific DNA...Ch. 15 - Prob. 5TYUCh. 15 - A cDNA clone contains (a) introns (b) exons (c)...Ch. 15 - Prob. 7TYUCh. 15 - Gel electrophoresis separates nucleic acids on the...Ch. 15 - A CRISPR locus in a bacterium contains (a) short...Ch. 15 - DNA molecular with complementary sticky ends...Ch. 15 - These highly polymorphic molecular markers are...Ch. 15 - Prob. 12TYUCh. 15 - Prob. 13TYUCh. 15 - Prob. 14TYUCh. 15 - EVOLUTION LINK DNA technology, such as the...Ch. 15 - SCIENCE, TECHNOLOGY, AND SOCIETY What are some...
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- Briefly describe how the CRISPR/Cas9 system modifies genomic DNA and why this can help treat genetic disease. Make it understandablearrow_forwardPlace the stages of a CRISPR-cas9 gene editing workflow in the correct order (there is one incorrect stage) Design a set of targeting sequences using algorithms. Clonal isolation Transfect cells Synthesise DNA insert oligonucleotides Purify plasmids Sequence Clonal characterisation (analysis of phenotype) Select for correctly gene edited cells (using antibiotic resistance and/or colour production for example) Add green fluorescent protein gene sequence into plasmid to aid selection of correctly transfected cells. Clone into CRISPR-cas9 expression vectorarrow_forwardDiagram the mechanism by which CRISPR-cas functions in the immune system of bacteria.arrow_forward
- Describe the basic components of CRISPR.arrow_forwardAfter sequencing a new genome for the first time, functional protein-coding genes are initially identified by: BLAST searches that look for viral integration in the host genome Bioinformatic searches that reveal consensus sequences in expressed genes Aligning expressed exons sequenced from cDNA using transcriptomics B and C A and Barrow_forwardSummarize the basic mechanism of how CRISPR-Cas9 can be used to edit a gene.arrow_forward
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