Biology (MindTap Course List)
11th Edition
ISBN: 9781337392938
Author: Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg
Publisher: Cengage Learning
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Chapter 15, Problem 3TYU
Summary Introduction
Concept introduction: DNA is a double-stranded molecule consisting of two strands of
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Recombinant DNA molecules are produced by incubating plasmids that have
been broken open by a restriction enzyme together with DNA molecules
containing complementary sticky ends in the presence of the enzyme ?
1) exonuclease
2) DNA connectase
O 3) DNA ligase
4) DNA polymerase
O 5) endonuclease
In Biotechnology, gene cloning is a very important technique. A vector is normally required
to perform this process. The vector commonly used to transform a bacterial host cell is the
plasmid.
(i)
State the THREE (3) important regions of the plasmid. Elaborate your answer.
In Cohen-Boyer’s recombinant DNA procedure, ___i___ must be used for both the bacterial DNA and the amphibian DNA ___ii___
a) the same restriction enzyme; so that the restriction sites are identical in the DNA of each species
b) different restriction enzymes; So that the genes outside the restriction site are maintained
c) different restriction enzymes; to ensure that the newly introduced genes are maintained in the bacterial DNA
d) the same restriction enzyme; to ensure that the newly formed DNA can replicate
Chapter 15 Solutions
Biology (MindTap Course List)
Ch. 15.1 - Prob. 1LOCh. 15.1 - Explain how gel electrophoresis is used to...Ch. 15.1 - Describe how PCR is used to amplify a specific...Ch. 15.1 - Compare the possible differences between a...Ch. 15.1 - Prob. 1CCh. 15.1 - Different forms of a protein are produced in the...Ch. 15.1 - What advantages does the PCR method have over gene...Ch. 15.2 - Describe the features of a typical CRISPR locus in...Ch. 15.2 - Explain the function of CRISPR in bacterial cells.Ch. 15.2 - Compare CRISPR-based endonucleases with...
Ch. 15.2 - Prob. 8LOCh. 15.2 - Prob. 1CCh. 15.2 - Prob. 2CCh. 15.2 - Prob. 3CCh. 15.3 - Prob. 9LOCh. 15.3 - Prob. 10LOCh. 15.3 - Discuss how qPCR, DNA microarrays (DNA chips), and...Ch. 15.3 - Explain how you would compare the expression of a...Ch. 15.3 - Prob. 2CCh. 15.4 - Describe how genome-wide association studies have...Ch. 15.4 - Explain how targeted gene silencing and knockout...Ch. 15.4 - Prob. 1CCh. 15.5 - Describe at least one important application of DNA...Ch. 15.5 - Prob. 1CCh. 15.5 - What are short tandem repeats (STRs), and why are...Ch. 15.5 - Why do gene targeting and mutagenesis screening in...Ch. 15.6 - Prob. 15LOCh. 15.6 - Prob. 16LOCh. 15.6 - Prob. 1CCh. 15.6 - Prob. 2CCh. 15.7 - Describe at least two safety issue associated with...Ch. 15.7 - What are some of the environment concerns...Ch. 15 - A plasmid (a) can be used as a DNA vector (b) is a...Ch. 15 - DNA molecules with complementary sticky ends...Ch. 15 - Prob. 3TYUCh. 15 - Which technique rapidly replicated specific DNA...Ch. 15 - Prob. 5TYUCh. 15 - A cDNA clone contains (a) introns (b) exons (c)...Ch. 15 - Prob. 7TYUCh. 15 - Gel electrophoresis separates nucleic acids on the...Ch. 15 - A CRISPR locus in a bacterium contains (a) short...Ch. 15 - DNA molecular with complementary sticky ends...Ch. 15 - These highly polymorphic molecular markers are...Ch. 15 - Prob. 12TYUCh. 15 - Prob. 13TYUCh. 15 - Prob. 14TYUCh. 15 - EVOLUTION LINK DNA technology, such as the...Ch. 15 - SCIENCE, TECHNOLOGY, AND SOCIETY What are some...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- An 9 kb circular plasmid is cut with the EcoRI restriction enzyme, and the reaction products are run on a DNA gel and stained with ethidium bromide. Bands of 1, 3 and 5 kb are seen. How many EcoRI sites are in the plasmid? Choose the one answer that is most correct. a) At least 2 b) At least 3 c) At least 1 d) None e) At least 4 f) At least 5arrow_forward16. Which of the following enzyme repairs the DNA backbone during molecular cloning (that is you are inserting the DNA fragment into a plasmid and the bases in the sticky ends are aligned but the backbone needs to be repaired). a) reverse transcriptase b) restriction enzymes c) DNA ligase d) polymerasearrow_forwardAmplified target regions of four different samples were separated using gel electrophoresis. DNA fragments labeled with the isotope P32 were separated by gel electrophoresis. The ultimate reason why unique target regions within each sample separate from each other in the gel is due to Amplified target regions of four different samples were separated using gel electrophoresis. DNA fragments labeled a) the amount of P32 bonded to each fragment. b) the number of restriction sites in each fragment. c) the distance between the primer recognition sites. d) the number of positive charges in each fragment.arrow_forward
- A. A plasmid is shown with the locations of various restriction enzyme sites labeled. If you cut the plasmid with Xhol and Xbal, which lane of the agarose gel represents the DNA fragments you would expect from the digestion? B. If you now decide to cut the plasmid with EcoRI, how many fragments will be produced and what will their sizes be? C. When running DNA samples on agarose gel, an electric field is applied. Towards which electrode will the DNA migrate and why?arrow_forwardIsolation of DNA is a crucial step for genetic engineering. (i) What is the difference between genomic DNA and plasmid DNA? (ii) Describe the common procedure for isolating genomic DNA without using the DNA extraction kit. ..1..arrow_forwardDEFINE THE FOLLOWING: 1) restriction enzyme 2) plasmid 3) recombinant DNAarrow_forward
- Considering the Polymerase Chain Reaction (PCR), we can state that: a) It is a technique that promotes linear amplification of the number of DNA molecules in a sample. b) Depends on an initial template which can be a DNA or RNA molecule c) The reaction must contain, in addition to the template sequence, nitrogenous bases, sense and antisense primers and DNA polymerase enzyme. d)The primer sense rings on the template strand 3’>5’, extending from the 3’ end e)arrow_forwardGel electrophoresis can be used for all of the following purposes except A) Determining the function of a gene product B Separating DNA (c) Assisting with constructing a restriction map (D) Determining the size of a DNA fragmentarrow_forwarda）What two restriction sites are you going to use to clone your PCR product into the pL4440 plasmid? What are their DNA sequence? b) State the primer sequence you will use to amplify the F27C1.7 gene ready to be cloned into the pL4440 plasmid? c) How would you go about cloning this amplified DNA into pL4440? Using your knowledge of cloning list 5 important aspects of the method.arrow_forward
- A small DNA molecule was cleaved with several different restriction nucleases, and the size of each fragment was determined by gel electrophoresis.The following data were obtained. (a) Is the original molecule linear or circular?(b) Draw a map of restriction sites (showing distances between sites) that isconsistent with the data given.(c) How many additional maps are compatible with the data?(d) What would have to be done to locate the cleavage sites unambiguouslywith respect to each other?arrow_forwardIf you wanted to create recombinant DNA using this enzyme(3' T A 5'), would you have to cut both samples of DNA with this enzyme, or could you use two different restriction enzymes? Explain.arrow_forwardIn Biotechnology, gene cloning is a very important technique. A vector is normally required to perform this process. The vector commonly used to transform a bacterial host cell is the plasmid. (i) State the THREE (3) important regions of the plasmid. Elaborate your answer. (ii) Besides plasmids, name TWO (2) other commonly used vectors in Biotechnology.arrow_forward
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