Concept explainers
a.
To determine: Whether, any of the suspects can be excluded as the one who committed the crime based on the results of the testing, or not.
Introduction: The genetic material of an individual is packed in the form of DNA. The evidence collected by the police is a small bloodstain from which the DNA is extracted and the fragments are separated with the help of electrophoresis. The separated fragments are transferred to a membrane where they are probed with radioactive DNA.
b.
To determine: The suspect which can be excluded as the one who committed the crime with a plausible reason.
Introduction: The genetic material of an individual is packed in the form of DNA. The evidence collected by the police is a small bloodstain from which the DNA is extracted and the fragments are separated with the help of electrophoresis. The separated fragments are transferred to a membrane where they are probed with radioactive DNA.
c.
To determine: Whether the pattern from the evidence is consistent with the band pattern of one of the suspects, or not and also determine the suspect with which it is consistent.
Introduction: The genetic material of an individual is packed in the form of DNA. The evidence collected by the police is a small bloodstain from which the DNA is extracted and the fragments are separated with the help of electrophoresis. The separated fragments are transferred to a membrane where they are probed with radioactive DNA.
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Human Heredity: Principles and Issues (MindTap Course List)
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardDNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?arrow_forwardDNA Profiles as Tools for Identification STRs are: a. used for DNA profiles b. repeated sequences present in the human genome c. highly variable in copy number d. all of these e. none of thesearrow_forward
- schematic diagram for the procedure in doing DNA extraction, isolation and quantificationarrow_forwardDescribe the characteristics of the extracted DNA, such as colour, shape, size, and consistency. Based on DNA Extraction experimentarrow_forwardWhich technique used to manipulate genetic material results in a significant increase in DNA or RNA fragments? Group of answer choices heat shock treatment electroporation gel electrophoresis polymerase chain reactionarrow_forward
- Explain the difference between a DNA profile and a microrarray.arrow_forwardYou are provided with coiled DNA and plasmid DNA that you subject to gel electrophoresis. Draw this gel. Remember to indicate the direction in which your DNA is moving and also show any reference samples. Also remember to show all components of your gel. Exact fragment sizes are not important.arrow_forwardBriefly explain the functions of the four procedures you learned about in this lab: DNA extraction PCR Gel electrophoresis DNA sequencing and analysisarrow_forward
- DNA fingerprinting uses a process called gel electrophoresis to separate the fragments of DNA. Once the DNA fragments are sorted, the pattern of bands can be analyzed. Gel Electrophoresis Procedure The smaller DNA fragments start to move away from the wells and the larger DNA fragments remain closer to the wells. An electric current is passed through the gel. DNA fragments are treated with a dye. A restriction endonuclease is added to the DNA. Using micropipettes, the DNA samples are added to the wells. DNA fingerprint is produced. DNA fragments are produced. The order in which a DNA fingerprint is produced using gel electrophoresis is Answer, Answer, Answer, Answer, Answer, Answer, and Answer.arrow_forwardWhy PCR needs the following: DNA template Primers DNA polymerase enzymes dNTPs Buffer Mg2+arrow_forwardExplain the basis for identification using DNA fingerprinting. – relate this to Microbiology not to human fingerprinting.Why does this technique work? Mention restriction enzymes and their function. Keep answer between 2-3 sentencesarrow_forward
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