Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 14, Problem 14.9PS
AMP-PNP Again. AMP-PNP (see question 14-5) can be used to study microtubule (MT) motors as well as myosins.
(a) What effects would you predict in a sperm flagellum to which AMP-PNP is added? In your explanation, please be specific about which molecule’s function would be inhibited and what the effect on overall flagellar function would be.
(b) When researchers incubated purified vesicles, axoplasm from squid giant axons, and MTs in the presence of AMP-PNP, the vesicles bound tightly to the microtubules and did not move. This observation suggested that AMP-PNP causes the formation of rigor complexes consisting of vesicles, microtubules, and microtubule motor proteins. To identify the putative motor, scientists then used AMP-PNP as a tool to cause the motor and MTs to bind tightly together. Then they collected MTs with bound proteins by centrifugation. The main protein purified in this way promotes movement of vesicles away from the cell body, where the nucleus resides. What was this protein?
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Need help, please. I know the answer isn't mitotic spindle or secretory and endocytic vesicle transport. I
think the answer is option C, axon stiffening, and neurotransmitter vesicle transport, but I am not 100%
sure.
What function of the microtubule motor proteins dynein and kinesin is
blocked by the use of colchicine to treat gout?
Select one:
O a. mitotic spindle
b.
secretory and endocytic vesicle transport
c.
axon stiffening and neurotransmitter vesicle transport
O d. ER, Golgi, and mitochondria movement
BONUS QUESTION! In neurons, the proteolytic enzyme y-secretase produces the Aß amyloid peptides shown below. The AB40 peptide is thought to play a protective role in the neuron. However, the AB42 peptide appears
to be toxic since it is found in the amyloid plaques that cause Alzheimer's Disease (AD). AB40 and AB42 are identical, except that AB42 contains two extra amino acid residues (shown in red) at the C-terminal end. Based on
your knowledge of amino acids and proteins, which of the following factors is most likely to explain the greater plaque-forming activity of AB42 compared to AB40?
Sequence of Aß40: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV
Sequence of AB42: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA
O The greater length of AB42 makes it more likely to aggregate and form plaques.
O AB42 has a lower pl than AB40, which makes it more likely to aggregate at physiological pH.
O AB42 is more hydrophobic than AB40, which makes it harder to clear from the cell and thus more likely to…
Please help me with this question. More than one answer may be correct. THe graph relating to the information is included below.
The figure shows the number of cells that have clusters of IRE1 molecules after those cells are treated with various levels of thapsigargin (Tg), a chemical that can induce ER stress. IRE1 can form these clusters when ER stress is induced and this clustering can cause activation of RNAse activity in IRE1. In this experiment, normal IRE1 was used (IRE1α) that can bind to Sec61, along with a modified version of IRE1 that binds to Sec61 more weakly than normal IRE1 (wIRE1α), and another modified version that binds to Sec61 more strongly than normal IRE1 (sIRE1α). From this figure you can conclude that:
Question 18 options:
IRE1 binding to Sec61 promotes the formation of IRE1 clumps
IRE1 binding to Sec61 prevents the formation of IRE1 clumps
co-translational translocation is a key process
the golgi aparatus is heavily involved in the unfolded protein response
The…
Chapter 14 Solutions
Becker's World of the Cell (9th Edition)
Ch. 14 - If myosin V moved more like an inchworm (meaning...Ch. 14 - Kinesins and dyneins are both microtubule-based...Ch. 14 - CONCEPT CHECK 14.2 Human sperm swim using a...Ch. 14 - A single myosin II motor domain can exert about 15...Ch. 14 - Prob. 14.4CCCh. 14 - In addition to being found at the rear of...Ch. 14 - Ciliobrevins. Ciliobrevins were reported in 2012...Ch. 14 - Prob. 14.2PSCh. 14 - Prob. 14.3PSCh. 14 - Rigor Mortis and the Contraction Cycle. After...
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