Becker's World of the Cell (9th Edition)
9th Edition
ISBN: 9780321934925
Author: Jeff Hardin, Gregory Paul Bertoni
Publisher: PEARSON
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Chapter 14, Problem 14.5PS
AMP-PNP and the Contraction Cycle. AMP-PNP is the abbreviation for a structural analogue of ATP in which the second and third phosphate groups are linked by an NH group instead of an oxygen atom. AMP-PNP binds to the ATP-binding site of virtually all ATPases, including myosin. It differs from ATP, however, in that its terminal phosphate cannot be removed by hydrolysis. When isolated myofibrils are placed in a flask containing a solution of calcium ions and AMP-PNP, contraction is quickly arrested.
- (a) Where in the contraction cycle will contraction be arrested by AMP-PNP? Draw the arrangement of a thin filament, a thick filament, and a cross-bridge in the arrested configuration.
- (b) Do you think contraction would resume if ATP were added to the flask containing the AMP-PNP-arrested myofibrils? Explain.
- (c) What other processes in a muscle cell do you think are likely to be inhibited by AMP-PNP?
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Chapter 14 Solutions
Becker's World of the Cell (9th Edition)
Ch. 14 - If myosin V moved more like an inchworm (meaning...Ch. 14 - Kinesins and dyneins are both microtubule-based...Ch. 14 - CONCEPT CHECK 14.2 Human sperm swim using a...Ch. 14 - A single myosin II motor domain can exert about 15...Ch. 14 - Prob. 14.4CCCh. 14 - In addition to being found at the rear of...Ch. 14 - Ciliobrevins. Ciliobrevins were reported in 2012...Ch. 14 - Prob. 14.2PSCh. 14 - Prob. 14.3PSCh. 14 - Rigor Mortis and the Contraction Cycle. After...
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