Chapter 12.3, Problem 37CQ

a.

Summary Introduction

To determine:

The characteristics that a person would expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes protein, DNA ligase.

Introduction:

Conditional mutations:

This mutationexpresses “mutant phenotype” only in certain conditions called “restrictive conditions”, and “normal phenotype” in other conditions called the “permissive conditions”. Example of conditional mutation: a temperature-sensitive mutation that expresses the “mutant phenotype” only at certain temperatures.

E. coli strain is isolated, and it contains temperature-sensitive mutations in its genes that encode different components for the replication process. The protein produced in each of the strain is contained mutated gene that is nonfunctional under the “restrictive conditions”. They are grown under “permissive conditions” and further rapidly enter to the “restrictive condition”. Under the restrictive condition, after one round of DNA replication, from each strain, the DNA is isolated.

b.

Summary Introduction

To determine:

The characteristics that a person would expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes. DNA polymerase I.

c.

Summary Introduction

To determine:

The characteristics that a person would expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes DNA polymerase III.

Introduction:

Conditional mutations:

This mutationexpresses “mutant phenotype” only in certain conditions called “restrictive conditions”, and “normal phenotype” in other conditions called the “permissive conditions”. Example of conditional mutation: a temperature-sensitive mutation that expresses the “mutant phenotype” only at certain temperatures.

E. coli strain is isolated, and it contains temperature-sensitive mutations in its genes that encode different components for the replication process. The protein produced in each of the strain is contained mutated gene that is nonfunctional under the “restrictive conditions”. They are grown under “permissive conditions” and further rapidly enter to the “restrictive condition”. Under the restrictive condition, after one round of DNA replication, from each strain, the DNA is isolated.

d.

Summary Introduction

To determine:

The characteristics that a person would expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes primase.

Introduction:

Conditional mutations:

This mutationexpresses “mutant phenotype” only in certain conditions called “restrictive conditions”, and “normal phenotype” in other conditions called the “permissive conditions”. Example of conditional mutation: a temperature-sensitive mutation that expresses the “mutant phenotype” only at certain temperatures.

E. coli strain is isolated, and it contains temperature-sensitive mutations in its genes that encode different components for the replication process. The protein produced in each of the strain is contained mutated gene that is nonfunctional under the “restrictive conditions”. They are grown under “permissive conditions” and further rapidly enter to the “restrictive condition”. Under the restrictive condition, after one round of DNA replication, from each strain, the DNA is isolated.

e.

Summary Introduction

To determine:

The characteristics that a person would expect to see in the DNA isolated from a strain with a temperature-sensitive mutation in the gene that encodes initiator protein.

Introduction:

Conditional mutations:

This mutationexpresses “mutant phenotype” only in certain conditions called “restrictive conditions”, and “normal phenotype” in other conditions called the “permissive conditions”. Example of conditional mutation: a temperature-sensitive mutation that expresses the “mutant phenotype” only at certain temperatures.

E. coli strain is isolated, and it contains temperature-sensitive mutations in its genes that encode different components for the replication process. The protein produced in each of the strain is contained mutated gene that is nonfunctional under the “restrictive conditions”. They are grown under “permissive conditions” and further rapidly enter to the “restrictive condition”. Under the restrictive condition, after one round of DNA replication, from each strain, the DNA is isolated.