Campbell Essential Biology with Physiology (6th Edition)
Campbell Essential Biology with Physiology (6th Edition)
6th Edition
ISBN: 9780134711751
Author: Eric J. Simon, Jean L. Dickey, Jane B. Reece
Publisher: PEARSON
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Chapter 11, Problem 4SQ

A eukaryotic gene was inserted into the DNA of a bacterium. The bacterium then transcribed this gene into mRNA and translated the mRNA into protein. The protein produced was useless and contained many more amino acids than the protein made by the eukaryotic cell. Why?

  1. a. The mRNA was not spliced as it is in eukaryotes.
  2. b. Eukaryotes and prokaryotes use different genetic codes.
  3. c. Repressor proteins interfered with transcription and translation.
  4. d. Ribosomes were not able to bind to tRNA.
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Why might a single base-pair mutation in eukaryotic mRNA be less serious than one in prokaryotic mRNA? a. If the mutation occurs in the 5' end of the start site, it will not affect the gene product. b. If the mutation occurs in the exon, it will not affect the gene product. c. If the mutation occurs in the splice site of a transcript with alternative splicing, only one gene product may affected. O d. If the mutation occurs in the intron or not in the splice site of a transcript with alternative splicing, it will nc affect the gene product. O e. If the mutation occurs in the 3' end of the start site, it will not affect the gene product. OLIE STIC N 1A
If eukaryotes have monocistronic genes, why is the number of known proteins more than the number of known genes?   A. Post-translational modification B. Alternative splicing C. Base substitution D. Post-transcriptional modification
What are two reasons that a eukaryotic gene (e.g. Green Fluorescent Protein in jellyfish) will not be expressed if it is inserted into a bacterial genome with no alterations? a. Bacterial genes do not contain introns b. Bacterial promoters have different consensus sequences than eukaryotic ones c. Transcription of this gene is affected by the simultaneous processes of transcription and translation d.
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Mitochondrial mutations; Author: Useful Genetics;https://www.youtube.com/watch?v=GvgXe-3RJeU;License: CC-BY