Your supervisor gave you a 5% saline (NaCl) solution. You need to make 100ml of 1.0% saline solution. How much 5% saline would you take to complete the task?
Q: Below is a trace from an analytical gel-filtration column upon which a single sample containing a…
A: Gel filtration column is used in gel filtration chromatography (GFC) technique. GFC is also known as…
Q: Using Benedict's Test would the substance (saliva + acid + potato chips) test positive or negative?…
A: Benedict's test is used for the identification of reducing sugars such as simple carbohydrates…
Q: how does the diameter of the column affect the flow rate of the elution in gel filtration…
A: Introduction Gel Filtration Chromatography IsA Type Of Size Exclusion Chromatography, Which Can Be…
Q: You were instructed to add 0.2 ml of kombucha to 9.8 ml of fresh tea, but instead added 2.0 ml of…
A: Dilution is defined as the ratio of stock volume to the final volume. Here, final volume is the sum…
Q: What is the relationship of concentration and absorbance based on the table and what are the changes…
A: The concentration of a solute in a solution can be determined spectrophotometrically by passing a…
Q: Explain how the following will affect blood film or smear a. Humidity b. Temperature c. Speed d.…
A: White blood cells (WBCs, leucocytes), red blood cells (RBCs, erythrocytes), and platelets can all be…
Q: The table below summarizes the results for the solubility test. Which solvent the lipid samples are…
A: The solubility of a substance in a solvent depends on the nature of the solute and the solvent.
Q: What will you do if you see bubbles forming on the gel after pouring it onto the gel caster/tray?
A: In laboratory, Electrophoresis is a technique for separation of the proteins and nucleic acid. The…
Q: How would you perform a 100-fold dilution, if you want the final volume to be 15mL? What volume of…
A: Dilution is the process of decreasing the concentration of a solute in a solution. To dilute a…
Q: Below is a BSA standard curve and the duplicate values of absorbance for 3 samples. Calculate the…
A: Lambert Beer law states that Absorbance of a solution is directly proportional to its concentration…
Q: Which of the following chromatographic techniques has an attached ligand on the stationary phase?…
A: Chromatography is a laboratory technique that is used to separate components, or solutes, of a…
Q: In Gel filtration chromatography, when will you stop collecting eluents if sample is not colored?
A: Gel-filtration chromatography is a type of partition chromatography that is used to separate…
Q: Why does a surgeon use sterile distilled water rather than sterile isotonicsaline to irrigate a…
A: Sterile distilled water comes under the category of hypotonic solutions. When our cells are kept in…
Q: How to prepare %25 100 ml Glucose solution?
A: Dextrose solution, also known as intravenous sugar solution, is a combination of dextrose (glucose)…
Q: if you are following a protocol requiring to use 200,000 cells, how much of the suspendion that…
A: Cells are the basic functional and structural unit of the body. The cells of the body are…
Q: What are the units used to measure cells?a. millimetersb. centimetersc. micrometersd. decimeters
A: As we know cells are so small that we can't see them through you are are naked eyes, for their study…
Q: What do you mean by the electroporation technique?
A: In microbiology and biotechnology, the new coding DNA is introduced to transform bacteria, yeast,…
Q: What percentage of the 15% saline solution is water?
A: A solution is a homogenous mixture of a solute and solvent. The solvent is the major part of the…
Q: What is electroporation and how does it work?
A: Electroporation: A microbiological technique, in which an electrical field is induced to…
Q: You perform a series of 4 different dilutions of 200 µL of sample into 800 µL of saline. What is the…
A: Dilution factor can be calculated using the formula:C1V1=C2V2
Q: If 10 µL of cells in suspension was mixed with 20 µL of a dye called trypan blue. DF = _____.…
A: According to the question, If 10 µL of cells in suspension was mixed with 20 µL of a dye called…
Q: tenry filled two jars with the same volume of water as shown in the picture. He placed a lid on J…
A: The experiments are conducted to understand biological and scientific phenomena that take place…
Q: The doctor ordered Ancef 225 mg IM every 8 hours. You have on hand a 1 gram vial with the…
A: Ancef contains Cefazolin. Cefazolin is a first generation Cephalosporin antibiotic that is used to…
Q: Normal room temperature is about 74 degree. What is the equivalent temperature on the Celsius…
A: Temperature defines the direction of spontaneous heat flow between objects/systems. Heat flows from…
Q: What happened when potato strip placed in salt water?
A: 1) A solution mixture that exhibits a higher concentration of salt than the surrounding medium. Then…
Q: Would a sample solution that has a high absorbance reading, have a low or a high concentration
A: Absorbance is a number that measures the diminishing of the transmitted radiant power in a solution.…
Q: How many milligrams of sodium chloride and lincomycin are required to prepare 100 mL of a 1%…
A: Delta Tf is freezing point depression ∆Tƒ of NaCl = 0.58 degrees celcius ∆Tƒ of Lincomycin = 0.09…
Q: what is the concentration of cells per 1 mL of solution?
A: Hemocytometer is also known as the cell counting chamber, is primarily used for counting cells…
Q: Viewing Object Through the Microscope: If the diameter of the low-power field is 1.5mm, an object…
A: “Magnification” is the process of enlarging an object. The microscopic magnification is used to make…
Q: What is electroporation technique?
A:
Q: (a) What two adjustments can be made to the condenser? (b) What effect do these adjustments have on…
A: Microscopes are optical instruments that use lenses to magnify an object. They help to observe…
Q: Using the sodium chloride equivalent method, make the following solution isotonic with respect to…
A: Any solute that dissolves will create an osmotic pressure when the solution is present across a…
Q: Compute for the sodium concentration of the patient. Use the same formula in the computation of the…
A: Spectrophotometry measures the absorbance spectrum of a substance. It measures the absorbance…
Q: rate for the remaining solution to complete on time. have infused. The set calibration is 20 gtt/mL.…
A: Given:- To give 1000 mL IV fluid to run for 8hrs. Only 250 mL infused after 4 hr Calibration set is…
Q: urine protein electrophoresis principle
A: Urine protein electrophoresis(UPEP) is a test used to determine the presence of protein in urine.It…
Q: You are asked to prepare Murashige & Skoog (MS) medium according to concentration shown the Table.…
A: We are having the concentration of the ammonium nitrate and potassium nitrate at 1 litre or 1000 ml…
Q: To prepare a vaginal irrigation, a nurse uses 3 tsp of vinegar and 1.5 L of water. a. How many ml…
A: Vaginal Irrigation is a technique wherein large amounts of water are flushed into the vagina in…
Q: In making red blood cell suspension, why do we need to wash red blood cells in saline?
A: Red cell suspension is a common reagent used for many serologic procedures. Red cell suspensions…
Q: How will you know when the endpoint of the titration is reached? Why is it necessary to mix the…
A: Reaction It is a process in which reactant molecules react to each other and form another molecule…
Q: You dilute 1 mL of bacterial culture with 99 mL of water. What is the resulting dilution factor?
A: Dilution factor:In order to calculate the Dilution factor, the final volume is to be divided by the…
Q: Let's say you had a 10mL, 25mL,50mL and a 100mL graduated cylinder.Which one would you use if you…
A: While working in laboratories, measurement of substances is an essential step. But of prime…
Q: Why can't we determine Vo, Ve and Vt for affinity chromatography?
A: Chromatographic techniques separate macromolecules based on size, ion exchange, or their affinity…
Q: He then ran the sample on an agarose gel and observed a strong band at approximately 400bp. What…
A: The band represent a small piece of DNA that was cut with restriction endonuclease and then…
Q: Which molecules, or function groups, or complexes that may be responsible for the absorbance at 595…
A: Spectrophotometry applications are useful to measure the absorbance, reflectance, and transmission…
Q: If FOV is 5 mm and the number of cells in FOV is estimated at 35, what is the cell size in mm? In…
A: Field of View = Field Number (FN) ÷ Objective Magnification.
Q: 0.9% salt (NaCl) concentration is isotonic to red blood cells. What would happen to a patient's red…
A: Osmosis is defined as the movement of water molecules from the higher the potential to the lower…
Q: drug is order 100mg/m2 po BID. How many milliliters will a patient receive if her BSA is 1.80m2 and…
A: Drug dose calculation is asked in this question. Ordered dose = 100 mg / m2 BSA of patient = 1.80…
Q: If the agar instructions say to mix 50 grams of agar in 1 liter of water, how many grams of agar…
A: 1 litre/ 1000 mL needs 50 grams of agar 1 mL needs 50/1000= 0.05 grams of agar 100 mL needs 0.05 X…
Q: how did you accurately get the volume for the three sample problems (COPD, NORMAL, and fibrosis)?
A: Lungs are essential parts of the respiratory system. A pair of lungs in humans are formed in such a…
Your supervisor gave you a 5% saline (NaCl) solution. You need to make 100ml of 1.0% saline solution. How much 5% saline would you take to complete the task?
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
- To the right is an image of a dilution that was performed. The volume above the top arrow indicates the volume that should be transferred to the next tube (i.e. Tube 1). The volume listed at the bottom-right of tube 1 indicates the volume of diluent that should be added to that tube. The stock concentration is 50μg/ml and you want to make a solution in tube 1 with a concentration of 0.4μg/ml and a total volume of 3ml. Stock ?ml Tube 1 ?ml a. How many milliliters of stock solution needs to be added to Tube 1? Round your answer to three decimal places (e.g. 0.111). b. How many milliliters of diluent needs to be added to Tube 1? Round your answer to three decimal places.Using 25 μL of serum, how much diluent would be needed to create a 1:2 dilution? ______ μLThe doctor ordered Oxacillin 650mg every 6 hours. The instructions on the 2gm vial states to reconstitute with powder with 11.5ml of sterile water for injection, yielding 220mg/1.5ml. How many ml will you administer? using dimensional analysis and show work
- The doctor ordered Pepcid 20mg. You have a vial labeled Pepcid 40mg/5ml. How many ml will you give?A dosage of 10mg is ordered. The solution strength is 2mg in 1 ml. How many ml will be administered? Round to the nearest tenth.Doctor ordered Haloperidol 3mg po bid. You have scored tablets containing Haloperidol 2mg. How many tab will you give?
- In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?1 2 3 1 point Mike needs to pipette 25 uL of solution. Which pipette should he use to pipette the most accurate amount of solution? P1000 OP20 1 point You need to pipette a solution of 150 uL. What pipette will you use? What will you set this pipette to (i.e. what will the numbers read on the pipette?) P1000; 0150 2000 P10 P200 ▶⠀ ⠀ > > n P10; 015 1 point Which of the following are best practices for using a micropipette? Select all that apply. Never exceed the upper or lower limit of the pipette If only pipetting a little extra, use a pipette outside of the range limit Point the pipette tip upwards Use a new tip for different liquids When withdrawing liquids, always release the plunger slowly P200; 150 P20; 150You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution; however, you only have access to two9 ml dilution blanks. Explain how you could accomplish this task with only two blanks.
- A pharmacist prepares 80 mL of an isotonic solution of 1.1 %w/v Lidocaine HCl (E-value 0.2). To prepare the solution, he dissolves Lidocaine HCl in purified water to make an isotonic solution. Then he dilutes the Lidocaine HCl solution with 0.9%w/v sterile sodium chloride to complete the formulation. How many milliliters of purified water is needed to make the formulation by this method?How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? V1= 0.005mL B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?If you transferred 15 mL of an undiluted sample into 35 mL of diluting fluid, what dilution have you created? (Note: provide your answer in decimal format to three decimal places.)