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In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?
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- You are observing the results from a standard plate count and you observe 53 CFUS. To make the plate count, you transferred 1 mL of a 10-4 dilution. How many CFU/mL are in the original sample? 1) 5.3 x 10-4 O 2) 5.3 x 103 O 3) 5.3 x 104 4) 5.3 x 105 O 5) 5.3 x 10-5If you plate 200 ul of a 1:100,000 dilution and get 123 colonies, what is the number of CFU/mL in the original sample?The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of culture to the first tube and mix. You get distracted, and transfer 0.1 ml to the third tube instead of the second. You perform the rest of the series as described.
- Below is an image of a dilution that was performed. The volume above each arrow indicates the volume being transferred to the next tube. The volume listed at the bottom-right of each tube indicates the volume of diluent added to that tube. .5ml 300ul 1ml stock Tube 1 Tube 2 Tube 3 3ml 4ml 8.5ml a. If the stock concentration is 50µg/ml, what is the concentration of tube 3 in ug/mL? b. What is the dilution factor of Tube 1?Observe the following Plate counts and then determine the correct number of CFU/ml Plate 1 = 564 colonies at 10^-5 dilution Plate 2 = 422 colonies at 10^-6 dilution Plate 3 = 317 colonies at 10^-7 dilution Plate 4 = 93 colonies at 10^-8 dilution 93 x 10^10 CFU/ml 9.3 x 10^-9 CFU/ml 93 x 10^9 CFU/ml 93x 10^8 CFÜ/ml 93x 10^-8 CFU/ml asap please0.1 ml of a blood sample is diluted into 9.9 ml. 0.1 ml of this is then diluted into 9.9 ml. 1 ml is plated. 42 colonies form. What is the CFUS/ml in the blood sample?
- An original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?What is the purpose of the RPR (RAPID PLASMA REAGIN ) Test? Why is a qualitative test performed before a semi-quantitative test? Why is it necessary to rotate the slide/card?A.How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?