what is the concentration of cells per 1 mL of solution?

Q: did water flow into or out of the plant cells (potato cells) in each of the samples examined? How do…

A: Osmosis is the movement of water molecules or solvent molecules from its high concentration towards…

Q: how does the diameter of the column affect the flow rate of the elution in gel filtration…

A: Introduction Gel Filtration Chromatography IsA Type Of Size Exclusion Chromatography, Which Can Be…

Q: Is the time required to Elute substance A, greater if N=1000 or N=2000?

A: N is number of theoretical plates in column Retention time (RT) is a measure of the time taken for a…

Q: What is measured at the A260 absorbance values?

A: DNA and RNA absorbs UV light due to heterocyclic rings of the nucleotides that is present in the…

Q: how many ml of sterile water injection must be mixed with two 4 ml vials of sterile hypertonic NaCl…

A: Tonicity refers to the ability of a solvent to cause cells to shrink or swell. A semipermeable…

Q: Describe how you would make 10mls of a solution with concentration: 10mM Glucose (MW-180.16g/mole)…

A: In a solution concentration of solute is reduced simply by mixing more water or by adding more…

Q: Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution…

A: Serial dilutions is a process of making step by step dilutions of a substance. It is formed by…

Q: What is the concentration of cells in the original cell suspension?

A: we can use the dilution formula:C1 V1 = C2 V2where:EXPLANATIONC1 is the initial concentration of the…

Q: You are instructed to perform the following three serial dilutions of a salt solution: 1:8, followed…

A: Dilution = initial volume / final volume Total dilution = multiply all the previous dilutions

Q: How do you calculate the amount of ammonium sulfate necessary to get a 20% solution?

A: Ammonium sulfate is the most commonly used for protein precipitation in protein purification.

Q: What will happen if cell inoculum density is more than 300

A: Introduction The population of microbes or cells injected into the fermentation medium, or any other…

Q: When doing a routine differential count for a CBC how many cells are counted?   a) 50 b 75 c 100…

A: CBC It is complete blood count which refers to the set of tests that gives the details of cells in…

Q: Why did the blank used in experimental procedures contain substrate, buffer and water?

A: A blank or blank determination is an analysis without a sample (Enzyme). Any enzyme assay requires a…

Q: You have a stock solution of a tubulin antibody. You were instructed by your teaching assistant to…

A: In laboratory research and experiments, accurate dilutions of solutions are often required to…

Q: You perform a series of 4 different dilutions of 200 µL of sample into 800 µL of saline. What is the…

A: Dilution factor can be calculated using the formula:C1V1=C2V2

Q: If you performed the serial dilution using 0.9ml of tapwater originally and mixed and transferred…

A: A serial dilution is one of the techniques which is useful in microbiology, serology, and other…

Q: Which contains more drug: 0.5 cc of 1% drug solution with 2 m of saline, or 0.5 cc of 1% drug…

A: The task at hand requires a comparison between the drug quantities in two distinct solutions. Both…

Q: You want to feed a new flask @: 2 x 105 cells/mL, 8 mL total per flask. Describe how you dilute your…

A: Viable or the live cells can be counted by the instrument called the hemocytometer. These live cells…

Q: Gel-filtration chromatography separates molecules according to their size . Smaller molecules…

A: Gel-filtration chromatography is a type of separation technique that uses a gel as a medium through…

Q: why is dtt added onto the total protein buffers?

A: Buffer is a solution that helps in maintaining the pH during a reaction. All proteins can act as…

Q: a) mentioned the name of simple laboratory method to roughly estimate the concentration of an…

A: Proteins have wide variety of essential function in our body like providing energy, structural…

Q: What are the effects of buffer solutions and what is its importance to life processes?

A: A buffer solution is an aqueous solution consisting of a mixture of a weak acid and its conjugate…

Q: What happens to pH of buffer solutions when small amounts of acid and base is added?

A: A buffer is a solution containing a strong acid and its conjugate base or a weak bases and its…

Q: Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that…

A: The countable range of colonies for an ideal plate count method is considered 30-300 colonies per…

Q: order: Pitocin(oxytocin) drip at 45 microgtt/min. The solution available is 20 units of Pitocin in…

A: To do this calculation , we must know there are 60 microgtt in 1ml.

Q: What is the function of the neutralization solution?

A: DNA Neutralizing Solution is utilized for the disengagement (isolation) of plasmid DNA by soluble…

Q: What is the role of each component of gel running buffer (TAE and TBE)?

A: Buffers are important for any biological reaction. DNA/RNA must be mixed with buffer for separation.…

Q: Which cell represents a hypotonic solution?

A: Answer: OSMOSIS : It is the movement of water from the higher concentration to the lower…

Q: Complete the table:   Solute Mass of solute Moles of solute Volume of solution Molarity of…

A: Here molar mass of MgSOu = 120.36 g/mol NaOH = 40 g/mol Ch3 oh = 32 g/mol

Q: What is a loading buffer and how does it function? What does 10x loading buffer mean?

A: Gel electrophoresis is a technique extensively used in molecular biology research to separate and…

Q: When loading samples onto a gel, why should expelling all the liquid in the tip be avoided?   a)All…

A: It is a laboratory tool commonly used in biotechnology and medicine to transport a measured volume…

Q: What are the uses of Tae Buffer and SDS buffer?

A: Buffers can be defined as the weak base and its salt or we can say weak acid and its salt. They…

Q: What is condition of cell placed in hypotonic solution?

A: Osmosis is a process in which water or solvent molecules move from lower concentration of solution…

Q: How many cells would you be plating if you pipetted 200uL into your dish from a 125 x 104 cells/mL…

A: The question is of calculation with keeping the units in our mind .

Q: Give only typing answer with explanation and conclusion What is the final concentration, as a ratio…

A: Medication is commonly administered in medical settings by diluting it in a solution, such as normal…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.