You are asked to prepare Murashige & Skoog (MS) medium according to concentration shown the Table. Answer the following question: 1. Prepare 250 mL stock solution (200X more concentrated) of ammonium nitrate and KNO3. 2. From the prepared ammonium nitrate stock solution above, calculate the volume required to prepare 200 mL MS medium.

If you diluted 200 uL of your culture into 1800 uL of water what is your dilution factor? (Show your work)

Propose a serial dilution that would give a dilution of 1/153 000 000. Each tube in your dilution series must have a volume of 50 ml or less.

Prepare the following LB media with antibiotic added. A 100ml of LB media with 25 micro/ml of Amp and 100micro/ml of Kan final concentration. A 100ml of LB is provided and amp of Kan stock at 100 mg/ml and 50 mg/ml are also provided. Find how much each antibiotic stock solution is needed to add 100ml of LB to reach desired antibiotic concentration.

Using absorbance readings and a standard curve relating absorbance to cell number, you suspect that a bacterial culture contains 7.4 x 109 CFUs/ml. What dilution of this sample would give you a countable plate?

100ml of LB media with 25 μg/ml of Amp and 100 μg/ml of Kan final concentration. You have 100ml of LB provided and Amp and Kan stocks at 100 mg/ml and 50 mg/ml provided. Determine how much of each antibiotic stock solution you need to add to 100ml of LB to reach desired antibiotic concentration.

How many mLs should be removed from your dextrose 70% bag?

A sample of Earth Alive Soil Activator was diluted by transferring 1 gram into a 9 ml dilution blank (tube A) and then serially diluted five (5) more times by transferring 1 ml of a previous dilution into a tube of 9 ml of sterile water. After making these serial dilutions, 2 ml samples were taken from tubes D, E, and F, added into empty petri dishes, and molten PCA was poured into each petri dish. Tubes A, B, and C were heated in a 80˚C water bath for 30 minutes, 2 ml samples of these tubes were added into empty petri dishes, and molten PCA was poured into each petri dish. These plates were allowed to solidify, and they were incubated at 35˚C for 48 hours. After the incubation period, you counted CFUs on all 6 plates. The results of your CFU counts are contained in table 1. Table 1. CFU counts for 3 plates from the heated dilutions (A-C) and the 3 plates from the non-heated dilutions (D-F) # CFUs   Not heated Heated A -- 400 B -- 45 C -- 6 D 350 -- E 31 -- F 2 --…

Explain the color changes observed throughout the peroxide value of oil experiment. Materials:1. Old and fresh oil samples2. Distilled water3. Acetic acid/chloroform solution4. Saturated potassium iodide solution5. Sodium thiosulphate (0.01M)6. 1% Starch indicator: dissolve 1g of starch in 10 ml of water and then add 90 ml of hotwater to it

Nitrate Reduction Test: in the document you submit, please describe the results for this test… (like yellow equals what?) 1) Give me the expected results for Pseudomonas fluorescens and Escherichia coli