rains of E. coli are isolated. They may be mutated on the ara, mal, leu and pro genes, only these 4 genes. The 5 strains (E1 to E5) are tested on 5 different media and onies are observed the next day. Rich Media (Lb) ES E4 ● E1 E3 E2Ⓡ MM + Mal+Leu E5 E4 X E1 E3 X E5 X MM + Mal E4 E1 E3 X E2 E5 E4 . MM + Mal+Leu +Pro E1 E3 E5 E2 MM + Ara E4 X X E1 E3 E2 X X onies are indicated by a black dot, absence of a colony is indicated by a cross. 1) Determine the genotypes of the 5 bacterial strains (E1 – E5)

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5 strains of E. coli are isolated. They may be mutated on the ara, mal, leu and pro genes,
and only these 4 genes. The 5 strains (E1 to E5) are tested on 5 different media and
colonies are observed the next day.
Rich Media (Lb)
E5
E4
E1
E2Ⓡ
E3
MM + Mal+Leu
E5
E4
X
●
E1
E5
X
MM + Mal
E4
X
X
E1
E3
E2
E5
✪✪
E2
E4
●
E1
E5
MM + Mal+Leu +Pro
E2
E3
MM + Ara
E4
X
X
E1
E3
E2 X
X
Colonies are indicated by a black dot, absence of a colony is indicated by a cross.
1) Determine the genotypes of the 5 bacterial strains (E1 - E5)
Transcribed Image Text:5 strains of E. coli are isolated. They may be mutated on the ara, mal, leu and pro genes, and only these 4 genes. The 5 strains (E1 to E5) are tested on 5 different media and colonies are observed the next day. Rich Media (Lb) E5 E4 E1 E2Ⓡ E3 MM + Mal+Leu E5 E4 X ● E1 E5 X MM + Mal E4 X X E1 E3 E2 E5 ✪✪ E2 E4 ● E1 E5 MM + Mal+Leu +Pro E2 E3 MM + Ara E4 X X E1 E3 E2 X X Colonies are indicated by a black dot, absence of a colony is indicated by a cross. 1) Determine the genotypes of the 5 bacterial strains (E1 - E5)
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