5 strains of E. coli are isolated. They may be mutated on the ara, mal, leu and pro genes,and only these 4 genes. The 5 strains (E1 to E5) are tested on 5 different media andcolonies are observed the next day.Rich Media (Lb)E5E4E1E2ⓇE3MM + Mal+LeuE5E4X●E1E5XMM + MalE4XXE1E3E2E5✪✪E2E4●E1E5MM + Mal+Leu +ProE2E3MM + AraE4XXE1E3E2 XXColonies are indicated by a black dot, absence of a colony is indicated by a cross.1) Determine the genotypes of the 5 bacterial strains (E1 - E5)

Microorganisms can grow in culture medium containing different nutrients one of those nutrients…

rains of E. coli are isolated. They may be mutated on the ara, mal, leu and pro genes, only these 4 genes. The 5 strains (E1 to E5) are tested on 5 different media and onies are observed the next day. Rich Media (Lb) ES E4 ● E1 E3 E2Ⓡ MM + Mal+Leu E5 E4 X E1 E3 X E5 X MM + Mal E4 E1 E3 X E2 E5 E4 . MM + Mal+Leu +Pro E1 E3 E5 E2 MM + Ara E4 X X E1 E3 E2 X X onies are indicated by a black dot, absence of a colony is indicated by a cross. 1) Determine the genotypes of the 5 bacterial strains (E1 – E5)

rains of E. coli are isolated. They may be mutated on the ara, mal, leu and pro genes, only these 4 genes. The 5 strains (E1 to E5) are tested on 5 different media and onies are observed the next day. Rich Media (Lb) ES E4 ● E1 E3 E2Ⓡ MM + Mal+Leu E5 E4 X E1 E3 X E5 X MM + Mal E4 E1 E3 X E2 E5 E4 . MM + Mal+Leu +Pro E1 E3 E5 E2 MM + Ara E4 X X E1 E3 E2 X X onies are indicated by a black dot, absence of a colony is indicated by a cross. 1) Determine the genotypes of the 5 bacterial strains (E1 – E5)

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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You have several different media onto which you inoculated eight strains of yeast (A-H). The media include a rich medium, an unsupplemented minimal medium, and minimal media each supplemented with one vitamin. Of the yeast strains, one is a prototroph and seven are auxotrophs for a vitamin. After overnight incubation, the following results were observed (tan patches represent growth): D plate 1 (A) B DE F GH plate 5 plate 4 plate 6 Which plate contains an unsupplemented minimal medium? [Select] Which plate contains a rich medium? [Select] plate 2 Which strain is a prototroph? [Select] Strain E is an auxotroph for niacin. Which plate reveals this specific auxotrophy? [ Select] plate 3 plate 7 One strain is an auxotroph for both choline and pantothenic acid. Which one is this most likely to be? [Select]
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A graduate student was assaying LD50 (lethal dose 50%) of two temperature-sensitive Francisella tularensis strains in HeLa cells (human cell line). Both strains can infect humans and cause fatal tularemia if untreated, but it is difficult to obtain LD50 values in human subjects. The data below shows LD50 (lethal dose 50%) values of the strains in human cell culture. Can you predict the more virulent strain of the two human pathogens? Francisella tularensis strain A: LD50 @ 20°C= 100; LD50 @ 37°C= 1000 Francisella tularensis strain B: LD50 @ 20°C= 1000 LD 50 @ 37°C= 100 O It is not possible to determine the virulence of the two strains as human pathogens from the provided data Strain A and strain B are equally virulent as human pathogens, as they average out in virulence. O Strain A is more virulent than strain A as a human pathogen. O Strain B is more virulent than strain A as a human pathogen.
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In the Avery, McLeod, McCarty Experiment where supernatant from heat killed, virulent S Strain pneumonia solutions were added to non-virulent R Strain pneumonia cell cultures and allowed to grow in liquid media (i.e., broth). In tubes where Protease was added to the supernatant prior to cell culture, what was the observed effect when plating and growing the S. pneumonia cells to solid media? Selected answer will be automatically saved. For keyboard navigation, press up/down arrow keys to select an answer. a b C d e All RNA was degraded and Transformation of the R Strain to S Strain occurred. All Protein was degraded and Transformation of the R Strain to S Strain occurred. All DNA was degraded and Transformation of the R Strain to S Strain occurred. All RNA was degraded and no Transformation occurred indicating RNA is the molecule of Transformation inheritance None of the above are true