You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information

Your animal cell culture laboratory suddenly suffered from a bacterial contamination. Several components like vitamins, yeast hydrolysate, and fetal calf serum are temperature sensitive and must be filtered through a bacteriological filter to add to the autoclaved animal cell culture media. After careful considerations, you found that there are no issues with the autoclaving, but there is something wrong with the filter sterilization process, and changing new batches of filter did not remove the bacterial contamination. Which of the following rationale best describes the probable cause? Group of answer choices A. The media is probably contaminated with E. coli,  which passed through the 0.2-micron bacteriological filter. B. The media is probably contaminated with Mycoplasma, due to the lack of cell walls they are the only bacteria that can pass through the 0.2-micron bacteriological filter.   C. All of the other answer choices could be correct D. The media is probably…

Which among A-D is false regarding growth and/or analysis of a bacterial culture? A) O Fed-batch growth: typically results in an increased yield of cells over that obtained with batch growth B) O Auxotroph: requires addition of specific growth factors in order to grow C) O Prototroph: typically the wild type strain that possesses all the features indicative of the species D) O None of A-D is false; all are true statements

You infect bacteria with two phages during a cis test. This produces a lysate that contains phage particles. Before you can count outcomes, you need to work out the amount of phage to use in subsequent experiments. You start by making two ten- fold serial dilutions of the lysate and then add 1mL of the final diluted lysate to a plate of permissive strain e.coli. You observe 10 plaques. How many phage per mL were present in the original lysate? a. 10 phage/mL o b. 3,300 phage/mL c. 10,000 phage/mL o d. 1,000 phage/mL e. 33 phage/mL