You mutagenized bacteria and isolated two mutants that you suspected to be defective in DNA synthesis. You measured incorporation of radioactivity (Y- axis) for various times (X-axis) at two temperatures (30 deg C shown as open circles and 40 deg C shown a filled circles in both figures) for the two mutants of bacteria (left and right figures). For this experiment, you can conclude that 20,000 1,000- 30° 0- 15,000- FAZ 10,000- 500 5,000- 0 40 80 0 40 80 120 120 Time (min) None of these conclusions You have isolated a component of RNA synthesis that is temperature insensitive O You have isolated mutants that are temperature insensitive O You have isolated temperature-sensitive mutants that are defective in DNA synthesis ³H-thymidine incorporated (cpm) 40°

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Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.