You mutagenized bacteria and isolated two mutants that you suspected to be defective in DNA synthesis. You measured incorporation of radioactivity (Y- axis) for various times (X-axis) at two temperatures (30 deg C shown as open circles and 40 deg C shown a filled circles in both figures) for the two mutants of bacteria (left and right figures). The results are shown in the accompanying figure. For the following statement, answer True or False.

The table below summarises the three stages of Meselson and Stahl's experiment and their results. (a) Complete the table by drawing, in the appropriate boxes, diagrams of the DNA molecules and mark the position and size of the DNA bands in the tubes. Experimental stage Diagram to show the strands in the DNA Position and size of DNA bands in the tube of molecules of the bacteria separating solution Stage 1 Bacteria grown for several generations in culture medium containing heavy nitrogen Stage 2 The bacteria from the end of stage 1 were grown for another generation in culture medium containing light nitrogen TA2G - Completed forms must be available for Open Awards extermal moderation purposes. Page 7 of 13 Stage 3 The bacteria from the end of stage 2 were grown for one more generation in culture medium containing light nitrogen (b) The bacteria at the end of stage two were grown for five more generations. After each generation, what would you expect to see in the test tube? Draw these…

In the transformation experiment by Griffith, two strains of an organism was injected into a mouse model. One, he labelled S was a heat-killed but pathogenic strain while the other, R was a non- virulent or non-pathogenic strain. Within 48 hours, the mouse died and only the S strain was obtained from the dead mouse. What conclusions can you derived from this experiment? A. Some of the pathogenic S strains survived, proliferated and subsequently overpowered the R strain. B. The R strains mutated and were converted to a pathogenic S strain. C. The R strains assimilated the DNA from the S strain and were transformed into the S strain. D. The R strain DNA hybridized with the S strain DNA such that the daughter DNA of the new generation of R all contained the S DNA. E. All of these conclusions are possible.

After mutagenesis of wild type Vibrio fisheri, you isolate two different mutant strains (A and B) that, unlike the wild type cells, fail to luminesce when grown to high density in a flask with appropriate medium. Curiously, however, when you inoculate both mutant strains in the same flask, you observe that the mixed (A+B) culture begins to emit light after growing dense. a) What gene/functions are likely affected in each of the two mutants? b) How does this explain their phenotypes?

PLEASE ANSWER BOTH PARTS OF THE QUESTION a.) The Question on the image b.) From this experiment, in how many genes have we identified mutations?

Watch the following video called "Human Genome Project Video" (https://fod.infobase.com/OnDemandEmbed.aspx?lti=1&token=30037&wID=97629&loid=0&w=400&h=300) then answer the following question in one paragraph each with evidence from the film to support your answers.  5) Answer in one paragraph and using the video provided to answer the following: There are companies now allowing people to clone their deceased pets. How do you feel about this? What are some good points and bad points to this service provided by some companies in your mind? 6) Answer in one paragraph and using the video provided to answer the following: There is rumor that humans are being cloned in other areas of the world. What ethical considerations can you think of that could be a problem with this type of medical experimentation? What is your opinion?

Can you please help with this question?

We isolate DNA from a bacteria that is wild type for the genes arg leu and his shear it in a blender mix it into a culture of bacteria that is mutant for the genes arg leu and his . We then plate on various media. These are the results: Media with arginine 20 colonies /ml Media with histidine 300 colonies /ml Media with leucine 500 colonies /ml Media with all three 10000 colonies/ml 21. What is this experiment called? A. transpiration B. conjugation C. transformation D. transduction

Select all that apply from the following to each transformation process : The following are White non-glowing colonies White glowing colonies Blue non-glowing colonies Blue glowing colonies None of the above The transformation are: a. LB + kanamycin plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain . b. LB + kanamycin plates that have been spread with the E. coli cells transformed with water, what phenotype of the colonies do you expect to obtain. c. LB plates that have been spread with the E. coli cells transformed with your pHSG298, what phenotype of the colonies do you expect to obtain. d. LB + kanamycin plates that have been spread with the E. coli cells transformed with PHSG298, what phenotype of the colonies do you expect to obtain. e. LB plates that have been spread with the E. coli cells transformed with your ligations, what phenotype of the colonies do you expect to obtain. f. LB plates…

Second, Question 2