You are testing a potential new antibiotic for its effectiveness at killing Streptococcus pneumoniae, the bacterium that causes pneumonia. As a first test of this drug, you grow the bacteria in the presence of different concentrations of this drug and analyze its effect on the growth of the bacteria. The data are shown in the table below listing the drug concentration in milliMolar (mM) against the percentage of growth relative to the control (no drug). Construct an appropriate graph for the presentation of these dat

You are testing a potential new antibiotic for its effectiveness at killing Streptococcus pneumoniae, the bacterium that causes pneumonia. As a first test of this drug, you grow the bacteria in the presence of different concentrations of this drug and analyze its effect on the growth of the bacteria. The data are shown in the table below listing the drug concentration in milliMolar (mM) against the percentage of growth relative to the control (no drug). Construct an appropriate graph for the presentation of these dat

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter10: Reconstitution Of Powdered Drugs

Section: Chapter Questions

Problem 7.2P

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You have an order for 1 gram of Cefazolin in D5W 100 ml. You have added 5 ml of sterile water to the 1 gram vial to reconstitute powder. However the recommended manufacturer’s diluent amount is 10 ml of sterile water for a final concentration of 100 mg/ml. How would reconstituting the vial with 5 mls affect the concentration and the final calculated dose. Please answer with explanation ASAP. I will really upvote. Thanks
You have an order for 1 gram of Cefazolin in D5W 100 ml. You have added 5 ml of sterile water to the 1 gram vial to reconstitute powder. However the recommended manufacturer’s diluent amount is 10 ml of sterile water for a final concentration of 100 mg/ml. How would reconstituting the vial with 5 mls affect the concentration and the final calculated dose?
Discuss in chemical detail the mechanism of action (how it works) of IMAC. Compare and contrast gel filtration chromotography with IMAC. Discuss the pros and cons for each technique. Why would a scientist prefer one technique over another? In what situations would one technique be more viable than the other?
Nutrient Agar (NA) is a general purpose medium used for the cultivation of a wide variety of non- fastidious microorganisms (Merck, 2000). Its ingredients are listed in Table 2. You are tasked to prepare 300ml of NA, determine the amount of ingredients and write in column 3 in Table 2. Show your calculations. *A sample calculation was made for you. Peptone = 300 ml x 0.5% = 1.5 g Table 2. Medium Composition of Nutrient Agar (NA) with the recommended proportions (Merck, 2019) Ingredients In % In Grams/300ml Peptone 0.5 *1.5 Meat extract 0.3 Agar 1.5 Distilled Water As needed
12:28 TABLE 1 Disk Symbol A B Today 10:42 AM INTERPRETING INHIBITION ZONES OF TEST CULTURES CHEMICAL METHODS OF CONTROL: ANTIMICROBIAL DRUGS Antimicrobial Agent Ampicillin when testing gram-negative bacteria Ampicillin when testing gram-positive bacteria Chloramphenicol Ceftazidime Carbenicillin Carbenicillin when testing Pseudomonas Cephalothin Ciprofloxacin Erythromycin Cefoxitin Sulfisoxazole Bunnsoxazo Gentamicin Conca companion Imipenem Penicillin when testing staphylococci Penicillin when testing other bacteria Polymyxin Rifampin Streptomycin Trimethoprim- Sulfamethoxazole (TMP-SMZ) Tetracycline Vancomycin when testing Staphylococcus spp. Vancomycin when testing enterococci PROCEDURE Second Period 1. Measure the zones of inhibition in millimeters, using a ruler on the underside of the plate (see Figure 3b). If the diameter is difficult to measure, measure the radius from the center of the disk to the edge of the zone. Multiply the radius by 2 to get the diameter of the zone. Disk…
Prepare the following LB media with antibiotic added. A 100ml of LB media with 25 micro/ml of Amp and 100micro/ml of Kan final concentration. A 100ml of LB is provided and amp of Kan stock at 100 mg/ml and 50 mg/ml are also provided. Find how much each antibiotic stock solution is needed to add 100ml of LB to reach desired antibiotic concentration.
Use the measurement tool to measure the zone of inhibition for the antibiotic sample in millimeters(mm) Each sample will need to be measured 3 times. Calculate the average for the antibiotic. Be sure to include units in your measurements.
We want to test different concentrations of an antibiotic to see how well it can kill bacteria on a petri dish. However, we have no idea how much (what concentration) of the antibiotic to use. Therefore, we are creating a dilution series in which we dilute the antibiotic 1:10 in PBS. Our stock solution of antibiotic is 200mg/mL and our stock concentration of PBS is 0.5M. Each petri dish will get 100uL of the diluted antibiotics. How would you make the dilution series? How many tubes would you need to make to test down to a final concentration of 20ug/mL antibiotic? HINT: PBS concentration is a distraction.
3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTES
In an experiment, you are treating cells in culture with drug X that was dissolved in The stock of drug X is 10 mM. The final treatment concentration needs to be 10 nM. You add the stock drug directly into 10 mL of cell culture media to reach the treatment concentration. How many ul of DMSO do you need to add to 10 mL of cell culture media for the vehicle control cells? 10 mL cell culture media with vehicle 10 mL cell culture media with 10 nM drug X Vehicle treated cells Drug treatment cells Drug X stock 10 mM
SHOW SOLUTION The rate of drug delivered to the skin by fentanyl transdermal patches is directlyproportional to the dimension of the patch. If a patch size of 5.5 cm? delivers12.5 mcg/hour of fentanyl, calculate the delivery rate of drug expected from a33-cm? patch.
On the graph that relates absorbance to flavin concentration, draw a horizontal line that starts at the point on the y-axis that corresponds to the absorbance of your unknown sample and ends at the curve you drew through your data points . Next draw a vertical line that starts at the point where the horizontal line intersected the curve and ends at the x-axis. The point where this vertical line intersects the x-axis corresponds to the concentration of flavin in your unknown sample. What was the letter on the tube with your unknown sample? What is the concentration of the flavin in your unknown