All of the following are valid measurements of growth except: A. Counting viable cells B. Estimating the weight of cells C. Determining the dry weight of cells D. Streaking for single colonies E. Measuring some cellular component F. Counting the total number of cells
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
All of the following are valid measurements of growth except:
A. Counting viable cells
B. Estimating the weight of cells
C. Determining the dry weight of cells
D. Streaking for single colonies
E. Measuring some cellular component
F. Counting the total number of cells
Use the general formula: [Number of colonies counted] × 10 × [how many times the sample must be multiplied to get to the original concentration: for example, 105] = a number of colony-forming units (CFU) per milliliter of starting culture.
Step by step
Solved in 2 steps
