Would heating the culture in a sealed, small-diameter tube that is totally immersed in a water bath produce more accurate results than the use of tubes that are partially submerged in a water bath? Give reasons for your answer.

Q: A glass flask is rinsed and autoclaved after use. It was then filled with sterile media in a sterile…

A: Introduction Microbes or microorganisms may be found on almost every surface, in the air we breathe,…

Q: If a culture had 4 cells to begin with and has a generation time of 60 minutes, how long would it…

A: "Microbiology" is the study of microorganisms and associated topics. Microbiology has gone a long…

Q: If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original…

A: CFU stands for colony-forming unit that gives rise to a particular colony. It is generally assumed…

Q: (1) What is the difference between specific growth rate and generation time?

A: As per our policy we are answering the first question only.  For the rest of the question kindly…

Q: How would you draw out a step in an experiment that included the following? Please help, I am so…

A: Horizontal gene transfer (HGT) is largely responsible worldwide for increasing the incidence of…

Q: In using an ultracentrifuge, arrange the following steps in correct order A. Turn the knob to the…

A: Centrifugation is a techniques in which involves centrifugal force to separate the particles from…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are…

A: Given,number of colonies in a 1mL of culture = 250. Also,given that this given solution is 100 times…

Q: Isolating a pure culture from a mix sample is typically done on solid agar but can be performed…

A: A microbial culture is a method of multiplying microbial organisms by letting them reproduce in…

Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…

A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…

Q: You make a 1:100 dilution of your culture and plated 0.1 ml of this sample on a Nutrient agar plate.…

A: The amount of organisms present in the original culture is called as colony forming unit per…

Q: Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution…

A: Serial dilutions is a process of making step by step dilutions of a substance. It is formed by…

Q: Why is it necessary to make pure subcultures of organisms grown from clinical specimens? How does…

A: Introduction: A subculture is developed by transferring several cells from an earlier culture to a…

Q: to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is…

A: Motility is the ability of an organism to move from one place to other, generally, the bacteria…

Q: A colleague performs a smear prep from a colony on an agar plate. Without staining it, he looks at…

A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…

Q: You inoculate a species of bacteria on a MacConkey agar which normally shows positive growth and the…

A: MacConkey Agar (MAC) is selective and differential agar that is used to isolate non-fastidious…

Q: Why would the optical tweezers be a method superior to dilution and liquid enrichment for obtaining…

A: Optical tweezers have been used to trap dielectric spheres, viruses, bacteria, living cells,…

Q: Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If…

A: Fibronectin is a glycoprotein found in the extracellular matrix of cells and is also obtained from…

Q: A microbiology student was given a mixed culture of two different gram positive bacteria species to…

A: In Microbiology, we grow bacteria on culture medium. Culture media contains specific mixture of…

Q: ake a look at your streak plate from the use of a mix culture. Does your plate show the results that…

A: The streak plate method is a microbiological technique used for the isolation of pure strain ;…

Q: ● . If the culture from which you are preparing the slide is a liquid, simply flame your loop, allow…

A: Introduction:- Cells are the basic structural and functional unit of life. Cells are capable of…

Q: Why after a certain period the growth rate of the culture decreases until growth stops?

A: Warmth, dampness, pH levels and oxygen levels are the four major physical and chemical components…

Q: o What is a pure culture? o Describe the procedure for making a streak plate from a clinical…

A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: Use the photo below to measure the diameter of each antibiotic (all 3) and record the name and…

A: The microorganisms or bacteria are grown in the culture plate in the microbiology laboratory for…

Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…

A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…

Q: How to isolate collagen from fish skin in the laboratory? Please write down each step in detail.…

A: Read some general instructions before starting the any scientific experiment. Maintain aseptic…

Q: Provide comparison and illustration of expected growth curves from a pure culture with the…

A: 1. The growth curve from a pure culture represents the number of live cells in a bacterial…

Q: Why is PBS buffer preferred when preparing a monolayer cell culture?

A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…

Q: - What is a pure culture? - Describe the procedure for making a streak plate from a clinical…

A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: which method do you think is more reilable, a test utilizing microbial growth media, or a…

A: The investigation of microorganisms is significantly aided by the ability to culture them, that is,…

Q: You perform a set of serial dilutions using 99-mL water blanks (successive 1-mL dilutions into…

A: Colony forming units (CFUs) are used to determine the concentration of viable microorganisms in a…

Q: In autoclaving media and glassware, why do you need to maintain the temperature at 121 0C and…

A: Microbial culture is done in sterile environments. The media and glassware used in the process are…

Q: You have a mixed culture containing 4 different organisms that you ihocl plate of media. When you…

A: Media or culture medium is a liquid or gel that is specifically designed to support the growth of…

Q: What is the advantage of the plating method over an electronic cell counting method in counting…

A: Bacteria culturing is done in order to grow the bacterial strains that are able to show resistance…

Q: Your animal cell culture laboratory suddenly suffered from a bacterial contamination. Several…

A: B. The media is probably contaminated with Mycoplasma, due to the lack of cell walls they are the…

Q: If a component is heat sensitive, how might you conveniently achieve sterility of that medium? a.…

A: Option A i.e. filtration

Q: Distinguish batch culture and continuous culture

A: Fermentation is used to convert carbohydrates into alcohol or acids. This process occurs in some…

Q: Examine the given table below. What interpretation can be drawn from the data regarding the test…

A: given here is a table that depicts phenol and a test disinfectant at various dilutions, following is…