You are growing up myoblasts C2C12 cells,to use in a myogenic study,You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2*107 cells.
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Q: ou are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks…
A:
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You are growing up myoblasts C2C12 cells,to use in a myogenic study,You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2*107 cells.
you seed a culture at 8 am on Monday with 5*105 cells .Assume all survive a generation time of 18 hrs and all cells are actively dividing.when will you expect them to be confluent?
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- You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm^2 and when confluent contains 2 x 10^7 cells. Question: You seed a culture at 8 am on Monday with 5 x 10^5 cells. Assume all survive, a generation time of 18 hours and all cells are actively dividing. When would you expect them to be confluent? A) 7:24 AM on Saturday B) 8 pm on Tuesday C) 8 am on Tuesday D) 9 pm on Thursday E) 12:48 pm on Wednesday F) They won't grow that longYou are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2 x 107 cells. 2) You seed a culture of squamous epithelial cells at 8 am on Monday with 1.2 x 105 cells. The next day at 8 am you harvest the cells. You know from past studies that the generation time is 26 hours for this cell line. How many cells do you predict you should have on hand? A) 1.64 x 10^5 cells B) 4.56 x 10^3 cells C) 5.23 x 10^10 cells D) 1.75 x 10^8 cells E) None of theseYou are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2 x 107 cells.You are growing up myoblasts 3)You seed another culture of a different end cell line, john12 cells at 8am on Friday with 104 cells. By Thursday at noon (12:00pm) they reach confluence. Assume the same confluency as stated above for T150 flask. What is the generation time (doubling time)
- You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm^2 and when confluent contains 2 x 10^7 cells. Question: You seed a culture of squamous epithelial cells at 8 am on Monday with 1.2 x 10^5 cells. The next day at 8 am you harvest the cells. You know from past studies that the generation time is 26 hours for this cell line. How many cells do you predict you should have on hand? A) 1.64 x 10^5 cells B) 4.56 x 10^3 cells C) 5.23 x 10^10 cells D) 1.75 x 10^8 cells E) None of theseYou are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2 x 107 cells.You are growing up myoblasts ) If 20% of a culture of human cells have a DNA content somewhere between 1&2*s (S-phase=8 hours) and 1X amount of DNA in non-dividing cells. What is the generation time?Before JCVI-syn3.0 was produced, what steps were used to create a synthetic Mycoplasma cell? Put the events below into the correct order. Drag and drop the events into the proper sequence from left to right. ▸ View Available Hint(s) First Step Transformed cells are detected by the presence of lacz, which causes them to cleave Xgal and produce a blue color. Yeast is transformed using Mycoplasma mycoides fragments. The synthetic chromosome is transformed into Mycoplasma capricolum. The synthetic Mycoplasma mycoides chromosome is purified. DNA fragments assemble through homologous recombination in yeast. Reset Help Last Step
- Which of the following are involved in microtubule stabilization or growth? Choose all that apply Side-binding MAPs CLASP Op18/stathmin Tau Kinesin-13 XMAP215 EB-1 << PreviousThe function of FtsZ was determined in part by using a temperature-sensitive ftsZ mutant. What is the phenotype of an ftsZ mutant when it is grown at 37°C? Cells of random sizes (some very small) are formed after cell division Normal sized cells are formed after cell division Cells do not divide, and turn into long filaments Cells die immediately upon shifting to 42°CTo design a drug that inhibits metastasis, which of the following strategies is the WORST idea? to disrupt cadherin complexes to inhibit Rho family proteins to inhibit ARP complex O to block myosin-based contractile structure
- Name the dark, linear, thread-like structures that you can see in some of the onion root tip cells. What Biomolecule of heredity do they contain?In Figure 10-25, why do only plant cells that have T-DNAinserts in their chromosomes grow on the agar plates?Do all of the cells of a transgenic plant grown from oneclump of cells contain T-DNA? Justify your answerYou seed another culture of different end cell line, Walker12 cells, at 8 am on Friday with 10^4 cells. By Thursday at noon (12:00 pm) they reach confluence. Assume the sae confluency as stated above for a T150 flask (culture area of 150cm^2 adn when confluent contains 2 x 10^7 cells). What is the generatoin time (doubling time)? A. 120 hours B. 80 hours C. 38 hours D. 8 hours E. 11.4 hours F. other ________