Descrive in 2-3 sentences an in vitro experiment that differentiates between the Arp2/3 complex’ ability to associate with both the side of the ‘mother’ actin filament and with the barbed end of the ‘mother’ actin filament. Your experiment should take advantage of capping protein (binds and blocks barbed ends), and direct visualization of actin filaments.

Descrive in 2-3 sentences an in vitro experiment that differentiates between the Arp2/3 complex’ ability to associate with both the side of the ‘mother’ actin filament and with the barbed end of the ‘mother’ actin filament. Your experiment should take advantage of capping protein (binds and blocks barbed ends), and direct visualization of actin filaments.

Anatomy & Physiology

1st Edition

ISBN:9781938168130

Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble

Publisher:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble

Chapter10: Muscle Tissue

Section: Chapter Questions

Problem 10RQ: According to the sliding filament model, binding sites on actin open when ________. creatine...

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Briefly describe an in vitro experiment that differentiate between the Arp2/3 complex’ ability to associate with both the side of the ‘mother’ actin filament and with the barbed end of the ‘mother’ actin filament. Your experiment should take advantage of capping protein (binds and blocks barbed ends), and direct visualization of actin filaments. Draw diagrams of actin filaments (simple lines are okay) that indicate the possible outcomes of your experiment. describe your interpretation of the possible outcomes for the mechanism of Arp2/3 complex-mediated branch formation
You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2 x 107 cells.You are growing up myoblasts ) If 20% of a culture of human cells have a DNA content somewhere between 1&2*s (S-phase=8 hours) and 1X amount of DNA in non-dividing cells. What is the generation time?
The Arp2/3 complex makes branched actin filaments by associating with a ‘mother’ actin filament and initiating assembly of a ‘daughter’ actin filament. Although it has been thought that Arp2/3 complex associates with and makes branches from the side of the ‘mother’ actin filament, it is alternatively possible that Arp2/3 complex associates with and makes branches from the barbed end of the ‘mother’ actin filament. Design an in vitro experiment (with purified proteins, such as actin and Arp2/3 complex) to determine which of these possibilities is correct by taking advantage of capping protein (binds and blocks barbed ends), and direct visualization of actin filaments. (1) Briefly describe your experiment in no more than three sentences (2) Draw diagrams of what the arrangement of actin filaments (simple lines are okay) are expected to look like in your proposed experimental and control conditions that indicate the possible outcomes of your experiment (3) Briefly describe your…
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You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2 x 107 cells.You are growing up myoblasts 3)You seed another culture of a different end cell line, john12 cells at 8am on Friday with 104 cells. By Thursday at noon (12:00pm) they reach confluence. Assume the same confluency as stated above for T150 flask. What is the generation time (doubling time)
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