You are developing a hypothetical culture medium for the isolation of salmonella Typhimurium. How will you use these unique characteristics to produce a culture medium that is both selective and differential for the isolation of presumptive Salmonella Typhimurium?
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You are developing a hypothetical culture medium for the isolation of salmonella Typhimurium. How will you use these unique characteristics to produce a culture medium that is both selective and differential for the isolation of presumptive Salmonella Typhimurium?
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- 1) In 2005 a typhoid outbreak in Delmas, South Africa, prompted the widespread testing of food and water sources to ascertain the origin of the outbreak. However, the origin of the outbreak could not be definitely tracesd. What could have been the possible reasons for this state of affairs? 2) A hypothetical culture media is being developed for the isolation of Salmonella Typhimurium. How will these unique characteristics be used to produce a culture media that is both selective and differential for the isolation of presumptive Salmonella Typhimurium?The isolation of Salmonella Typhimurium and S.Typhi require different protocols: A) In what aspects do these protocols differ? B) why do these protocols differ? C) How will you be able to distinguish between these organisms on XLD and Hektoen Agar?1) What are all of the different colony types that can be seen on a mixed-culture streak plate? 2) What kinds of colonies can be seen when mixed cultures of E. coli, M. luteus, and Serratia marcesens? 3) What is the range of colonies that may be seen with each mixed culture?
- Streptococcus pneumoniae is a Gram-positive bacterium that colonizes the mucosal surface of the upper respiratory tract in humans. The presence of this bacterium in the nose and throat is widespread in the population, and in most people, colonization with Strep. pneumoniae is asymptomatic. The figure attached shows a comparison of in vitro growth curves of the wild-type strain of Strep. pneumoniae, as well as a Strep. pneumoniae mutant strain with a defect in one bacterial gene. The graph on the right shows the growth curve following addition of lysozyme during the logarithmic phase of bacterial growth. Which statement could account for the data in these graphs? Strain B is wild-type Strep. pneumoniae, and strain A is a mutant that cannot modify its peptidoglycan to be lysozyme-resistant. Strain B is wild-type Strep. pneumoniae, and strain A is a mutant that that expresses increased levels of LPS. Strain A is wild-type Strep. pneumoniae, and strain B is a mutant that cannot modify its…1) based on the difference in appearance between mixed culture streak plates and broth culture plates, what would lead you to believe that you have created a pure culture when you inoculated the broths? 2) when using the loop dilution technique to produce pour plates, did all the plates produce isolated colonies? If your goal was to ultimately create pure cultures of E. coli, M. luteus, and S. morcescens, which plate or which dilution would you use, and why would you use this plate? 3) What are the advantages and disadvantages of streak plating compared with pour plating?1) both streak plating and pour plating produce isoluated colonies. What is the underlying explanation for why both methods work; that is, what are both methods doing with repect to the bacterial cells? 2) If streak plates failed to produce isolated colonies, describe two things that you could do to improve your chance of generating isolated colonies. 3) Why do we care so much about producing isolated colonies? what is an isolated colony composed of? What can you do with an isolated colony?
- How can you tell that these S. aureus isolates are different strains?McConkey agar red is considered to be both differential and selective for the isolation of members of the family Enterobacteriaceae. Explain whyyou are given a mixed culture of s. aureus, E. coli and P. aeruginosa. how would you isolate each other from this mixed culture?( Besides using plate technique)
- Why is aseptic urine collection important when cultures are ordered? If you counted 20 colonies from a 0.01-ml inoculum of a 1:10 dilution of urine, how many organisms per milliliter of specimen would you report? Is this number significant? What can you learn from visual inspection of a urine specimen? How would you relate these to the microorganisms present in the sample? How are UTIs acquired/transmitted? Explain why E. coli is frequently implicated in cystitis in females.Which among A-D is false regarding growth and/or analysis of a bacterial culture? A) O Fed-batch growth: typically results in an increased yield of cells over that obtained with batch growth B) O Auxotroph: requires addition of specific growth factors in order to grow C) O Prototroph: typically the wild type strain that possesses all the features indicative of the species D) O None of A-D is false; all are true statementsa) write down the isolation protocols of salmonella Typhi and salmonella Typhimurium. (b) list 5 biochemical characteristics in which S.Typhimurium BIOCHEMICALLY differs from S.typhi.