Which of the following would suggest your stock culture is contaminated? Gram stain indicates Gram-positive bacilli. Broth media become turbid. Gram stain indicates both Gram-positive cocci and Gram-negative bacilli. Colony morphology is uniform on the streak isolation plate. Gram stain indicates Gram-positive staphylococci.

Describe characteristics of Proteus Vulgaris in the Agar: (How does colonies look like (color) and explain does it grow on that agar.  Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient Agar

Gardnerella vaginalis is a gram variable rod. What type of media should be used when culturing for it? Describe the appearance of the colonies.

During the preparation of a smear leading into simple staining of the bacterial culture S. epidermidis you forgot to heat fix the slide. What would you see on this slide as compared to a slide that was properly prepared? Please explain.

Microorganism Type of Microorganism Gram Stain Shape Tryptic Soy Agar McConkey Agar Blood Agar EMB Agar Mannitol Salt Agar Bacillus cereus                   Bacillus megaterium                 Bacillus subtilis                   Candida albicans                 Escherichia coli                   Micrococcus luteus                 Neisseria sicca                   Proteus vulgaris                 Pseudomonas aeruginosa                 Serratia marcescens                 Staphylococcus aureus                 Staphylococcus epidermidis

Which of the following reports bacteria counts as colony-forming units? Turbidity Most probable number Standard plate count Microscopic counts

Explain why some cells are pink and others are the Gram- stained bacterial smear. Add references and source

A microbiologist sets up a serial dilution to analyze bacteria in raw milk.   Tube 1 – 0.1 mL milk diluted in 9.9 mL sterile water Tube 2 – 0.1 mL milk from Tube 1 diluted in 9.9 mL sterile water Tube 3 – 1.0 mL milk from Tube 2 diluted in 9.0 mL sterile water   What is the dilution of raw milk in Tube 3?

A microbiologist is working with a pure strain of bacteria isolated from a mixed sample. Which of the following would indicate that the bacterium is NOT Helicobacter pylori?     Cells have flagella when viewed by flagellar staining.     Secretes acids produced from metabolism of sucrose.     Cells are curved rods.     Produces and secretes urease enzyme.     Cells stain Gram negative.