A veterinarian suspects that a new disease of house cats is caused by a specific type of bacterium. Describe a set of procedures for providing this hypothesis. EXAMPLE GIVEN ON THE IMAGE.
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A veterinarian suspects that a new disease of house cats is caused by a specific type of bacterium. Describe a set of procedures for providing this hypothesis. EXAMPLE GIVEN ON THE IMAGE.
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- 20. Starting with the MacConkey agar and IMVIC tests. Describe how a bacterium can be biochemically identified as Escherichia coli.1. An undercooked boiled egg was received in the microbiology laboratory with a request culture and identification. This egg was served in a pack lunch during a birthday party. All guests who ate this egg were brought to the hospital for vomiting and dehydration. Some had bouts of diarrhea. The signs and symptoms were short-lived after treatment was received. The egg was homogenized in tryptic soy broth. This was plated in sheep’s blood agar plate and MacConkey agar plate. After 24 hours of incubation, the following observations were noted: Sheep’s blood agar plate: 4+ (many) beta hemolysis, creamy white colonies MacConkey agar plate: no growth The gram stain result of the isolate yielded gram-positive cocci, mostly in clusters. If Staphylococcus aureus is suspected, list 3 tests that will be most relevant in its identification – i.e. to differentiate it from other gram-positive cocciThe world is facing a Corona Virus (COVID-19) Pandemic and as a potential scientist and a microbiology student, you have been required to prepare the easiest and most affordable hand sanitizer that can be utilized by students in various microbiology laboratories. Each student in your class has been given 96% ethanol and you are required to prepare 70% ethanol hand sanitizer in a 2L spray bottle. Describe clearly the method you will use in order to achieve the aim of the practical.
- 1. Describe the three different type of hemolysis that are observed on blood agar.2. What is a selective medium?3. What is a differential medium?4. Which media can be used to isolate E. coli samples from contaminated lettuce?5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea?6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain.7. What is the color or TSI for Salmonella?8. What is a fastidious organism?1. Which, if, any of the drugs would you prescribe for a somewhat effective treatment of a patient infected with this test organism? 2. Which, if, any of the drugs would you not prescribe because they were ineffective in killing or inhibiting a patient infected with this test organism? 3. Briefly explain how you would determine if one of the above drugs was broad spectrum or if it was narrow spectrum.1. Please discuss how the Methyl Red-Vogues Proskauer test help distinguish microbes from each other.
- 1. What is the most common sterilization technique used in laboratories? 2. List at least 5 procedures of the aseptic technique and describe its uses. 3. Why is Gram stain one of the most important and widely used stains in bacteriology? 4. Explain the Gram staining technique in chronological order. Indicate the reagent used and the time of usage on each reagent.The first step for directly linking a microbe to a specific disease according to Koch's postulates is to O isolate microbes from the blood of healthy animals. obtain a sample of blood or other body fluid from a diseased animal. O culture the blood or other body fluid from a discased animal using nutrient medium. O inject a sample of blood or other body fluid from a diseased animal into a healthy animal. O compare the blood of a sick animal to blood obtained from a healthy animal.1. What is the critical threshold between bacterial infection and simple bacterial contamination? 2. Why is aseptic technique important in urine collection? 3. Give at least 5 bacteria that can cause Urinary Tract Infection.
- 3. The bacterial isolated from the abscess was also used in the test shown below. (a) What is the name of this test? (b) What are you testing for? (c) Describe how you would evaluate the results of this type of test. (d) Based on the results of this test as shown below, should the doctor and patient be concemned? Explain! 4. What organism could be causing the patient's abscess? Explain your reasoning. Focus Mode + 100% D Focu DA 07:33 PM R. W 11/18/2020 0 日 9 search1.application of the technique and viral technique that employs to immunofluorenscence test5. biovars Match each of the options above to the items below. collection of strains that share stable characteristics progeny of a single, pure microbial culture strains with physiological or biochemical differences strains with differences in physical appearance strains characterized by antigenic properties