What colony characteristics can be used for differentiation of bacterial species? As an example, compare the properties of colonies of Serratia marcescens and Micrococcus luteus on your streak plate.
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What colony characteristics can be used for differentiation of bacterial species? As an example, compare
the properties of colonies of Serratia marcescens and Micrococcus luteus on your streak plate.
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- 1) both streak plating and pour plating produce isoluated colonies. What is the underlying explanation for why both methods work; that is, what are both methods doing with repect to the bacterial cells? 2) If streak plates failed to produce isolated colonies, describe two things that you could do to improve your chance of generating isolated colonies. 3) Why do we care so much about producing isolated colonies? what is an isolated colony composed of? What can you do with an isolated colony?What is the importance of describing the morphological characteristics of bacterial colonies when cultures were sampled from an environmental source? Explain and justify your points.How can colony morphology be used in the identification of bacterial species? What are the environmental factors that affect the growth of bacteria? What is agar (or agar-agar)? Is this different from nutrient agar? Why? How is xylol used to clean the lens of a microscope? What may happen if xylol is constantly used for this purpose? For what purpose would you adjust each of the following microscope components during a microscopy exercise? iris diaphragm coarse adjustment knob fine adjustment knob condenser mechanical stage control
- Draw a 4-inch circle. Draw out the streaking pattern used to isolate single colonies on a plate.The process of collecting characteristics of your unknown bacterium can be helped by the use of a dichotomous key. A dichotomous key is a flowchart that can be used to identify an organism. a) in your own words explain how to create and use a dichotomous key to identify unknown bacteriaIn the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: The primary stain, crystal violet, is dark purple The counterstain (safranin) causes the purple color to develop Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcohol Crystal violet is fixed inside the cell as a CV-Iodine complex once iodine is added None of the other four answers (All are reasons that gram-positive bacteria are purple
- Bacillis brevis are lysed using a valve-type homogenizer. The extent of disruption depends on the applied pressure and the number of passes through the homogenizer chamber and can be described by the equation, ln(1 − ?) = −????. Is Bacillis brevis a gram-positive or gram-negative bacterial cell? Based on this response, describe the cell wall structure and how it differs from the other type. Develop an equation for calculation of pressure as a function of the number of passes through the homogenizer. What factor(s) did you take into consideration when selecting the number of passes? Escherichia coli cells are lysed with the same homogenization, is this organism gram-positive or gram-negative? What impact does increasing the value of the constant, a, have on the required number of passes at a given pressure? What factors impact the value of a? If fine cell debris after homogenization makes subsequent solid-liquid separation difficult, what would you recommend? Answer as…In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT: None of the other four answers (All are reasons that gram-positive bacteria are purple Crystal violet is fixed inside the cell as a CV-lodine complex once iodine is added The counterstain (safranin) causes the purple color to develop The primary stain, crystal violet, is dark purple Thick peptidoglycan in the gram-positive cell wall prevents decolorization by alcoholAll of the following are correctly matched and can be found in bacteria EXCEPT: None of the other four answers (all are correctly matched and can be found in bacteria) Nucleus membrane-bound organelle containing multiple chromosomes Ribosomes - site of protein synthesis Cell wall contains peptidoglycan Cell (plasma) membrane - encloses cytoplasm; phospholipid bilayer with proteins
- 1.What colony characterisitics can be used for differentiation of bacterial species? As an example, compare the properties of colonies of Serratia marcescens and Micrococcus luteus on your streak plate. 2.Define the term colony as it relates to bacterial growth on solid media? 3. why is dilution a necessary part of pure culture preperation? 4.What advantage(s) does the streak-plate method have over the pour-plate method?Identify the cellular morphology for this Gram stain. What is the Gram reaction? What is the bacterial shape? What is the bacterial arrangement? What is the staining dye? [Choose ] [Choose ] Cocci Singles no arrangement Pleomorphic Chains Gram-negative Crystal Violet Phenol Red Bacilli Gram-positive Clusters SafraninPlease describe the pros and cons of the following methods for counting bacterial cells. 1) Petroff-Hausser counting chamber 2) Coulter Counter 3) Plate Count