Why are Gyrase inhibitors good antibiotics to use therapeutically? O DNA Gyrase is required by prokaryotes, but not eukaryotes, to complete chromosomal replication so it's a good target to inhibit. ODNA Gyrase is required by eukaryotes, but not prokaryotes, to complete chromosomal replication so it's a good target to inhibit. It's impossible to develop resistance against Gyrase inhibitors so they are always effective. ODNA Gyrase is required by both prokaryotes and eukaryotes to complete chromosomal replication so it's a good target to inhibit.

Which of the following statements are TRUE? (Multiple Select - choose ALL that are TRUE) A bacterial cell has all of the genes necessary to generate a pillus, it just needs activation by an F plasmid O After conjugation, the cell that started out F- becomes F+ O Conjugation cannot occur between an F+ cell and an R+ cell O Conjugation cannot occur between an F+ cell and an F- cell Conjugation can occur between two strains even if the two strains are blocked from touching by a filter. O Most antibiotics block bacterial conjugation O After conjugation, the cell that started out F+ becomes F- O Conjugation cannot occur between an F+ cell and an F+ cell

Part ii. ) please

Which statement about plasmid is FALSE: Plasmid is nonessential DNA in the bacteria Plasmids replicate independent of the genome replication. Their size can vary from a few kilobases to a few hundred kilobases. They are all circular.

Conjugation results in the transfer of genetic material between two bacterial cells, but only occurs if one bacterial cell is O F+ Conjugated Transformed O F-

Producing Insulin Many diabetics are treated using recombinant insulin. Place the following steps involved in producing insulin in order. 1 | Gene is expressed in the bacteria to make large amounts of insulin 2 E Identify the gene for insulin 3 | Clone the bacteria with the recombinant plasmid 4 Insulin inserted into a plasmid Inserted plasmid into bacterial cell 6 | Use restriction enzymes to remove insulin gene LO

How would a scientist, observing a prokaryote and a eukaryote replicating their DNA, be able to distinguish the two? Group of answer choices Prokaryotic replication does not require a primer. Prokaryotic chromosomes have a single origin of replication, while eukaryotic chromosomes have multiple origins of replication. DNA polymerases of prokaryotes can add nucleotides to both 3' and 5' ends of DNA strands, while those of eukaryotes function only in the 5' --> 3' direction. DNA replication in prokaryotic cells is conservative. DNA replication in eukaryotic cells is semi-conservative.

You have two DNA samples. Only one was expected to have plasmid DNA that was isolated from it. Both DNA samples were exposed to two different treatment conditions: untreated, or treated with DNase I. How should the DNase I treatment change how DNA appears on the gel?

Draw a diagram showing what pGEM will look like after it has been digested with BamHI. Be sure to show both strands (with enzyme sequence) and include the origin of replication, the ampicillin resistance gene, 3’ and 5’, and the DNA sequence at the restriction site. It will probably be easiest to simply draw it as an open circle, not a line. Now show what pGEM will look like after it has been digested with EcoRI instead of BamHI. Again, be sure to show both strands and include the origin of replication, the ampicillin resistance gene, 3’ and 5’, and the DNA sequence at the restriction site.

Practice Case study p. 224 part: Tuberculosis (TB) in indigenous populations. Lily was a recent science graduate of the University of Alberta and was 11 months into her 12 - month Master of Science in Global Health degree, at Dalhousie University. This relatively new program was created to provide the multidisciplinary training required of scientists working to meet the complex challenges of global public health issues. The University had partnered with the National Collaborating Centre for Indigenous Health (NCCIH) and that was why Lily now found herself doing her Global Health Field Practicum in the Northern Territories, working on a study of drug resistant TB infections among Innuit communities. The different communities included in her research were close to 7000 individuals, with multiple diagnosed cases of TB. And while she was grateful that drug-resistant TB in Canada remained below the global average (Public Health Agency of Canada, 2019), she was cognizant of the possibility…