Proteins at work at the replication fork - Topoisomerase Replication fork movement Helicase - Next Okazaki fragment ill start here. - RNA primer Primase - RNA primer Okazaki "fragment - Single-strand- binding proteins B clamp- DNA DNA Leading strand polymerase III dimer polymerase I Lagging strand - Ligase FIGURE 7-20 The replisome and accessory proteins carry out a number of steps at the repication fork. Topoisomerase and helicase unwind and open the double helix in preparation for DNA replication. When the double helix has been unwound, single-strand-binding proteins prevent the double helix from re-forming. The ilustration is a representation of the so-called trombone model (named for its resemblance to a trombone owing to the looping of the lagging strand) showing how the two catalytic cores of the replisome are envisioned to interact to coordinate the numerous events of leading- and lagging-strand replication. LaunchPad ANIMATED ART: Loading- and lagging-strand synthesis

Proteins at work at the replication fork - Topoisomerase Replication fork movement Helicase - Next Okazaki fragment ill start here. - RNA primer Primase - RNA primer Okazaki "fragment - Single-strand- binding proteins B clamp- DNA DNA Leading strand polymerase III dimer polymerase I Lagging strand - Ligase FIGURE 7-20 The replisome and accessory proteins carry out a number of steps at the repication fork. Topoisomerase and helicase unwind and open the double helix in preparation for DNA replication. When the double helix has been unwound, single-strand-binding proteins prevent the double helix from re-forming. The ilustration is a representation of the so-called trombone model (named for its resemblance to a trombone owing to the looping of the lagging strand) showing how the two catalytic cores of the replisome are envisioned to interact to coordinate the numerous events of leading- and lagging-strand replication. LaunchPad ANIMATED ART: Loading- and lagging-strand synthesis

Name: Referring to Figure 7-20, answer the following questions:a. What is th
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Description: Referring to Figure 7-20, answer the following questions:a. What is the DNA polymerase I enzyme doing?b. What other proteins are required for the DNApolymerase III on the left to continue synthesizingDNA?c. What other proteins are required for the DN

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter28: Dna Metabolism: Replication, Recombination, And Repair

Section: Chapter Questions

Problem 17P

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Match the components of bacterial replication and transcription correctly. v [Choose ] Topoisomerase Il helicase loader RNA polymerase must accumulate enough for DNA replication to begin Single stranded binding protein sigma factor proteins of the RNA polymerase adds nucleotides to RNA transcript DnaA bacterial helicase DNA gyrase - needed for recognition of promoter site in transcription Beta clamp DNA polymerase Tau complex two alpha units, beta, beta prime, and omega Dnac [ Choose ) DnaB ( Choose]
Alternative splicing Template strand S F yIGUide1,mq00:c-00: Replisome Transforming principle Origin of replication (or)eleb al msxS ain Coding strand Transcription factors Leading strand Single nucleotide polymorphism Okazaki fragment Telomerase M Nucleoside RNA Polymerase I RNA Polymerase II RNA Polymerase IIIon & of qu 9ven UoY Insertion mutagenesis Spliceosome Transcription Unit SNP Reverse transcriptase 1 Seminal work by Oswald Avery and colleagues demonstrated that DNA is what Frederick Griffiths called this etniog OS dotsM bioW 1-2kb of newly synthesized DNA strands are called this ainiog PS Assembly of the replisome is an orderly process that begins at these precise sites Snoiteeu 4 Transcribes ribosomal RNA genes in eukaryotes 5. A large nucleoprotein complex that coordinates activity at the replication fork Single base pair differences between homologous genomic regions isolated from different members of a population Complex of proteins and snRNAs catalyzing the removal of…
nd 2 minutes): Any RNA polymerase in any organism: O A Synthesizes RNA chains in the 3 to-5" direction O B. Binds tightly to a reqion of DNA located thousands of base pairs away from the transcobed rogion of the DNA OC Has proofreading activity O D. Separates DNA strands throughout a long region of DNA (up to tinousands of base pairs) and then copies one of them. OE Has a subunit called A (lambda), which acts as a proofreading ribonuclease OF. Can initiate synthesis of a new RNA chain without a primer
to 4 minutes) The schematic diagram below shows organization of the DNA replication fork. Match parts of the diagram (labeled A-F) with the corresponding term from the answer list (designated 31 parental duplex 5' 3' fork progression v A 1Lagging strand 2. An Okazaki tragment 3.Site of action of DNA topoisomerase 4 Leading strand 5. Site of action of DNA helicCase 6.Site of action of DNA ligase
DNA Replication Topoisomerase LAstand Primase E strand D Match the diagram with the correct term listed below. Structures Letter Okazaki fragments DNA polymerase RNA primer Helicase Leading strand Lagging strand 3' 5'
AUG Met ACG ANALYSIS ANALYSIS: A DNA strand undergoes all the process included in the central dogma. The DNA strand used as a template is given below: Parent strand DNA: 5-AGA-ACT-AAA-СТА-ТCG-СТT-CGT-3 DNA daughter strand: hnRNA: MRNA: original protein: mutated MRNA: mutated protein: second letter G UCU UUU Phe UAU Tyr UGU UGC Cys UAA stop UGA stop| A UAG stop UGG Trp UUC UCC UAC Ser Leu UCA UUG UUA UCG G CUU CCU CAU CGU His CAC CAA CUC C CGC Leu CUA Pro Arg A ССА CGA Gln CAG CUG CCG CGG AUU ACU AAU AUC lle A AUA AAC Asn AAA AGC AGA AGU Ser ACC Thr АСА AAG LyS GAU ASP AUG Met ACG AGG Arg G GUU GCU GGU) GUC GCC GAC GGC Val GUA GCA Ala GAA Gly GGA A Glu GUG GCG GAG GGG G Translating the unmutated MRNA that was obtained after splicing, the original protein sequence will be? The original protein is [A] (For your answer, use the one-letter symbol (all caps) for the amino acid residues separated by dashes, e.g. C-A-S-H). first letter third letter
Where are the Okazaki fragments found? 5 Lagging Parental Strand Topoiso- merase Helicase Single Stranded Binding Proteins Frank Boumphrey M.D. 2009 Leading Parental Strand Replication Fork Forming O just before the DNA ligase and after the DNA helicase just before the DNA helicase and after the DNA ligase In the leading strand in the lagging strand Which is the first step in replication RNA Primase lays down RNA primer for new nucleotide bases. DNA polymerase builds new strands by bring in new nucleotide bases. O DNA Helicase unwinds original DNA strand O Ligase knits together fragments on lagging strand Which is the first last step in replication RNA Primase lays down RNA primer for new nucleotide bases. DNA polymerase builds new strands by bring in new nucleotide bases. O DNA Helicase unwinds original DNA strand O Ligase knits together fragments on lagging strand O O OO O O O O O O O O
art B-Processes occurring at a bacterial replication fork The diagram below shows a bacterial replication fork and its principal proteins. Drag the labels to their appropriate locations in the diagram to describe the name or function of each structure. Use pink labels for the pink targets and blue labels for the blue targ Replaces RNA primers with DNA nucleotides. Catalyzes phosphodiester bond formation, joining DNA fragments. Leading strand Breaks hydrogen bonds, unwinding DNA double helix. Synthesizes DNA 5' to 3 on leading and lagging strands. Lagging strand Coats single-stranded DNA, preventing duplex formation. Relaxes supercoiled DINA. Synthesizes RNA primers on leading and lagging strands. e Overall direction of synthesis reset ? help
5-ccuaaucg-34 3'-acctgcctataccggattagetetgatectaagcatgtc-5 The diagram above shows an RNA primer hydrogen-bonded to a DNA template. Which letter indicates the site where DNA polymerase would add nucleotides to this structure? OA OB D Any of these is possible C
Match the enzyme on the left with its role in DNA replication DNA polymerase I helicase DNA ligase DNA polymerase III topoisomerase primase 72 W w# 3 E $ 4 R % 5 T A 6 MacBook Pro Y & 7 U * 8 replaces primers with DNA connects Okazaki fragments to form a continuous strand of DNA synthesizes short RNA fragments used to initiate DNA synthesis Uses the 3'OH of an RNA primer to synthesize the leading strand and Okazaki fragments keeps DNA from getting tangled up ahead of the replication fork "unwinds" the DNA double helix at the origin and replication forks 1 ( 9 X 0 0 P + 11 Next
What is the mRNA coded by this template DNA strand? - DNA Template Strand: 3' ATGGTACGGTCG 5' TACCATGCCAGC 5' O 3' TACCATGCCAGC 5' O5' AUGGUACGGUCG 3" O5' UACCAUGCCAGC 3' UACCAUGCCAGC 5'
Multiple Replication Forks in E. coli II On the basis of Figure 28.2, draw a simple diagram illustrating replication of the circular E. coli chromosome (a) at an early stage, (b) when one-third completed, (c) when two-thirds completed, and (d) when almost finished, assuming the initiation of replication at oriC has occurred only once. Then, draw a diagram showing the E. coli chromosome in problem 3 where the E. coli cell is dividing every 20 minutes.