An investigator obtains a bacterial temperature-sensitive mutation that affects a step in the process of DNA replication at 42°C but not at 30°C. She grows the cells at 30°C and, upon shifting the temperature to 42°C, she discovers that Okazaki fragments accumulate in unusually large quantities.  What is the likely target affected by the temperature-sensitive mutation?   primase DNA polymerase III DNA helicase DNA Ligase DNA gyrase

Q: polymerase with 5'-3' as well as 3'-5' exonuclease and proofreading activity is Select one: a.…

A: The error-correcting processes used in the genetics is known as the proof reading. During the…

Q: Select all of the following that need to be in a polymerase chain reaction that will successfully…

A: Polymerase chain reaction (PCR) is a molecular technique used by scientists to multiply particular…

Q: Polymerase chain reaction is— 
     a reaction involving a chain of different types of polymerases…

A: PCR is a technique to make many copies of DNA in vitro. It relies on thermostable Taq polymerase and…

Q: Why should the extracted DNA be immersed in TE buffer

A: Introduction DNA( deoxyribonucleic acid) the natural chemical of complicated molecular structure is…

Q: 1000bg 800b 500bd 300bd 100bg Identify the sizes of the DNA bands in the agarose gel above using the…

A: Agarose gel electrophoresis is a separation technique mostly used for DNA molecules. Where a sample…

Q: DNA polymerase I DNA polymerase II DNA ligase Primase RNA primer 5' Lagging strand 3' 3' Okazaki…

A: The process of copying of double stranded DNA by the application of several Enzymes and proteins…

Q: Does the addition of a histidine tag affect DNA polymerase activity and or processivity? Give a…

A: DNA polymerase is an important enzyme of dna replication and can add nucleotide triphosphates to 3’…

Q: In ONE sentence define the function of the following 1. SSBS = The SSBS are single 2. Beta subunit…

A: These are all the enzymes used in replication of DNA Replication of DNA is a very important process…

Q: Which of the following enzymes has 5’ to 3’ exonuclease activity? DNA ligase DNA polymerase I…

A: To shift DNA strands, the 5′ to 3′ exonuclease activity can be used with the polymerization…

Q: Eddie the E. coli bacterium cell is suspicious that his DNA contains a thymine dimer. What is the…

A: Here in the question it is described that DNA thiamine dimer is formed. And it's proposed that DNA…

Q: Which Strict Operating Requirements DNA Polymerase Has?

A: DNA polymerase is an enzyme involved in the synthesis of nucleotides. These are important for the…

Q: What was the function of each solution or step in the DNA purification protocol? The following…

A: Deoxyribonucleic acid extraction is the isolation and purification of DNA from sample using a…

Q: What is the role of alcohol in extracting DNA? DNA is a polar molecule with an overall negative…

A: DNA stands for deoxyribonucleic acid, that is a molecule that contains the directions an organism…

Q: DNA ligase

A: The correct option is Option 3. DNA ligase

Q: A 3000 bp circular DNA was treated with both HindIII and EcoRI restriction enzymes. EcoRI cuts at…

A: In this question, we are given two restriction enzymes EcoRI and HindIII with their restricting…

Q: Which of the folllowing is the term used for ultra compact form of DNA coiling? a. supercoiling…

A: Compacting DNA is important for packaging it within the cells. As the length of a DNA molecule can…

Q: Choose the right combination of components required to set up a polymerase chain reaction from the…

A: Polymerase chain reaction is a method widely used to rapidly make millions to billions of copies of…

Q: Which of the following statements regarding Nucleotide Excision Repair (NER) and Base Excision…

A: Base excision repair is the mechanism of repair of DNA which has been damaged and this repair occurs…

Q: Treating the DNA samples with heat will break the hydrogen bonds holding the molecules together.…

A:

Q: A 1% by weight agarose gel is typically used in agarose gel electrophoresis. If you used a 2%…

A: During agarose gel electrophoresis, an agarose solution is prepared, it is cooled to prepare a gel,…

Q: Which technique used to manipulate genetic material results in a significant increase in DNA or RNA…

A: In biotechnology laboratories different techniques are used to manipulate the genome of the…

Q: What is the name of the process of rehybridization of melted DNA? replication denaturation annealing…

A: DNA hybridization is the process, in which two strands of the complementary DNA are joined to form…

Q: An RNA-dependent DNA polymerase that carries the RNA template with it to synthesize repeats at the…

A: Replication is the process of duplication of the DNA. Replication is initiated by recognizing the…

Q: The epsilon subunit of DNA polymerase III is responsible for its _______ activity. A-5'---->3'…

A: DNA Polymerase III is an enzyme that is involved in DNA replication and synthesis. It has 3'-5'…

Q: Enzymes that catalyze DNA denaturation or melting are:   gyrases   topoisomerases   helicases…

A: DNA is a genetic material that is a double stranded structure polymer of nucleotides. DNA…

Q: Primers are composed of and are made by the enzyme DNA; helicase DNA; primase O RNA; primase O RNA;…

A: A primer is a single strand of DNA that is short and acts as a starting point for the synthesis of a…

Q: An Investigator obtains a bacterial temperature-sensitive mutation that affects a step in the…

A: Most of the replication enzymes are proteinaceous in nature. The structure of a protein is formed by…

Q: i the polymerase chain reaction, the DNA denaturation step requires a emperature in the range of ?°…

A: SOL1 In humans, fertilization is the fusion of a human egg or female gamete and sperm or male gamete…

Q: You have two tubes, each with a pair of DNA fragments inside them. Tube #1 has fragments that are…

A: Electrophoresis  Electrophoresis is used in biological laboratory to separate different size of DNA,…

Q: The nucleotide sequence below is one half of a double stranded DNA sequence. The highlighted portion…

A: DNA molecule is the genetic material present in the animals. Genetic material  is present in the…

Q: . The DNA polymerases are positioned over the following DNA segment (which is part of a much larger…

A: Introduction  DNA replication is very crucial process for the continuation of life as every new…

Q: Why is the use of temperaturestable DNA polymerase an important factor in the polymerase chain…

A: The polymerase chain reaction (PCR) is the process of artificially synthesizing multiple copies of a…

Q: The following gel was produced in manual DNA sequencing. What would the unknown DNA template be that…

A: Answer :- In Sanger manual sequencing, DNA of interest is amplified along with dye-labeled chain…

Q: Mutation of subunit epsilon (ɛ) of DNA polymerase II will result in an E. coli strain that has a…

A: Question -Mutation of subunit epsilon (ε ) of DNA polymerase lll will result in an E. coli strain…

Q: Which of the following subunits of DNA polymerase has proofreading ability?

A: Introduction: The hereditary material which transfers from parents to offspring is DNA. It is stored…

Q: Discuss the following statement: “primase is a sloppy enzyme that makes many mistakes. eventually,…

A: Enzymes are biocatalysts that aid in the speeding up of reactions. They are proteins that help to…

Q: Which of the following DNA Repair systems is most likely to be activated after someone goes to a…

A: DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA…

Q: proteins are denatured and eliminated during the DNA extraction process. how can we determine the…

A: Deoxyribonucleic acid (DNA) is the hereditary unit of life, which carries the genetic information in…

Q: Why is DNA not soluble in ethanol whereas other components cells are soluble. What is the rationale…

A: DNA isolation is a method through which the cell is lysed and DNA is isolated from the same using…

Q: Which of the following is the correct order in extracting the DNA?   I. Washing of DNA II. Tissue…

A: DNA act as genetic material in most of organism . It is two stranded , ladder like structure . DNA…

Q: For the following short sequence of double stranded DNA and the given primers, there will be one…

A: Polymerase chain reaction (PCR) is generally used to produce millions or even billions of copies in…

Q: After four cycles of PCR, which type of PCR product predominates? Explain why.

A: Polymerase chain reaction (PCR) is a method widely used for rapidly producing millions of copies of…

Q: In DNA isolation techniques, a washing step is always done prior to the final resuspension. What is…

A: DNA isolation: The process of extraction of DNA may be broken down into five primary phases that…

Q: In order to complete the strand on the left, the following are needed: DNA polymerase [Select]

A: DNA replication is very important and critical process taking place in all living cells in their S…

Q: Why Sanger sequencing uses ddNTPs and how they differ functionally from dNTPs. Does Sanger…

A: Sanger sequencing is the method of sequencing (determining the sequence of DNA) of DNA using the…

Q: We usually think of enzymes as being most active at around 37°C, yet in PCR the DNA polymerase is…

A: PCR, like DNA replication in an organism, necessitates the use of a DNA polymerase enzyme to create…

Q: A segment of DNA is isolated and added to a 01 mixture of four deoxynucleotides, two specific…

A: The correct option is D.

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.