Which one of the following is NOT considered a biohazard? old bacterial cultures slides containing bacterial gram-stains tissues that were used to blow your nosed. used gloves
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Which one of the following is NOT considered a biohazard?
- old bacterial cultures
- slides containing bacterial
gram-stains - tissues that were used to blow your nosed.
- used gloves
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- A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 CelciusOn which of the following types of media would you expect untransformed bacteria to grow on? LB agar LB agar with ampicillin Untransformed bacteria will not grow on any type of medium. Untransformed bacteria will grow on all three types of media. LB agar with ampicillin and arabinoseBefore viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the following usually needs to happen (and why)? Staining (to increase magnification). Heat, radiation or antimicrobial chemical treatment (to kill the bacteria for safe observation). Add immersion oil (to increase resolution). Add a cover slip (to reduce contamination). The viewing chamber needs to be flushed of air (to create a vacuum).
- MALDI-TOF, is a method for identifying bacteria quickly. Look at this graphic then put the steps in order. Please follow a pathway of steps involving the Bacterial Culture: Get a readout of species identification and (sometimes) whether it is resistant or susceptible to antibiotics. Use colony from a nutrient agar plate. Prepare the sample, run it through MALDI-TOF machine. Grow a clinical sample on nutrient agar.Why is a Bunsen burner used in the microbiology lab? (select all that apply) The flame is used to sterilize the inoculating loop. The flame kills microorganisms that float in the air and could contminate samples. To sterilize the lid of the agar plate. To reduce the spread of antibiotic resistance.A culture medium that does not grow gram-positive organisms would be a differential plate enrichment plate selective plate streak plate pour plate
- You have 2 mL of bacterial culture. How would you make a 10^-2 dilution using the entire culture volumeThe first step in identifying an unknown bacterium is to __________. The first step in identifying an unknown bacterium is to __________. culture your unknown on selective media perform endospore staining on your culture prepare a pure working stock simple staining on your culture acid-fast staining on your cultureTrue or False: An isolated bacterial colony supplies cells.to grow "pure culture" of the bacteria. True False
- It is called a "Negative Stain" because the stain has a negative charge, and is therefore: repelled by the bacterial surface attracted by the bacterial surface able to penetrate the bacterial surfaceYou are working through the Gram Staining procedure on a mixed culture of S. aureus (G+) and E. coli (G-) (gram + & gram -) organisms respectively. If you accidentally use too much alcohol / acetone during the procedure, which of the following will likely happen? E. coliE. coli will appear to be Gram + will appear to be Gram +E. coli will appear to be Gram + S. aureus will appear to be Gram - Both organisms will appear colorless Both organisms will appear purple None of the answers are correctThere is definitely survival of some microorganisms even after alcohol treatment. Without staining or microscopic scrutiny, predict what types of microbes are growing on the medium where you made the right thumb impression after treatment.