You are tasked with quantifying bacteria in your soil sample. You resuspend 1.0g of soil in 10 ml 1x PBS, then do a 10-fold serial dilution of your soil sample. You plate 150 µl onto each plate. The 104 dilution plate contains 175 colonies after 2 days of incubation, how many bacteria do you estimate were present in your initial soil sample? (# colonies/volume plated in ul) * dilution factor * ul convert to ml * volume suspended soil (ml/g) = Read the formatting instructions below before entering your answer! 1. Make sure your answer is in correct scientific notation using an "e" to indicate the exponent! For example, 2500 would be 2.5e3, 0.0066 would be 6.6e-3. 2. For answers greater than 1, round up to the nearest tenth. For example, 2515 would be 2.5e3 and 51510 would be 5.2e4.

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Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.