If you have a mixed (Gram positive and Gram negative) sample, and you complete a Gram stain, and all the cells are purple at the end of the stain, what have you done wrong? forgotten safranin forgotten crystal violet forgotten decolorizer forgotten iodine everything worked perfectly

Q: Explain the reasons you would use a negative stain?

A: In a negative stain the slide is actually stained. This is because the negatively charged stain…

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: Describe how cyclin-dependent kinases (Cdks) are regulated and explain how they help cells pass…

A: The cell cycle is the lifespan of the cell from the time it is formed until it divides into two…

Q: You look at a slide you prepared and, despite the fact that you know you put organism onto the slide…

A: Gram-staining is a laboratory test that detects the presence of bacteria and also helps to…

Q: Why is heat necessary in spore staining?

A: BASIC INFORMATION  SPORULATION  It is a process during which spores are formed.  We can find it in…

Q: what color would you expect for a gram positive microorganism for each incorrect mistakes while…

A: "Gram staining" is a technique used in microbiology. It is a method in which a bacterium can be…

Q: Why is the Gram stain considered a differential stain?

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

A: Introduction: Acid-fast staining is a type of differential staining technique that was first…

Q: A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C…

A: Introduction  Capsule is the outer most covering, which is present even outside the cell wall. This…

Q: Which of the following statements is FALSE?   Question options:   stains increase the contrast of…

A: 3rd statement is false i.e the chromophore of an acidic dye is positively charged.

Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…

A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…

Q: Move the images of bacterial cells into the chart to illustrate the color of gram-positive and…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: I need to discribe the "the colors of gram+ and gram (-) bacteria" in each independent step of the…

A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…

Q: Choose the 4 most correct steps from the list below for a simple positive stain and organize them…

A: The above question is related to the laboratory technique of bacterial staining. Staining is a…

Q: Why is oil immersion necessary to see bacteria in the laboratory?

A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…

Q: Acid-Fast stain M. Smegmatis -morphology shape -Arrangement -Acid-fast or non-acid-fast

A: Introduction :- Mycobacterium smegmatis is a rod-shaped bacterium that is commonly used as a model…

Q: Makes Twenty sentences in English and Spanish related to mechanics

A: The objective of this question is to create twenty sentences related to the field of mechanics, each…

Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…

A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…

Q: Why is it sometimes difficult to attain both good resolution and good contrast at the same time for…

A: Magnification and resolution are important because the magnification in larges the image and the…

Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…

A: To answer this question first, we need to understand what is gram staining and what are the steps of…

Q: Explain the process of transamination in the muscle, transport of ammonia to the liver and…

A: The amino acid is transaminated to produce a molecule of glutamate. Glutamate undergoes oxidative…

Q: Which stain is used 1st after you heat fix the fly during the gram staining procedure

A: Gram staining is the procedure by which the gram positive and gram negative bacteria can be…

Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)   a…

A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…

Q: Why is it important to perform a gram stain for any type of bacteria? After performing the gram…

A: The gram staining is a procedure that is useful in classifying the bacteria based on retention of…

Q: What are the advantages and disadvantages of gram staining

A: Introduction  Gram staining is a widely used laboratory technique in microbiology to differentiate…

Q: Gram E. coli 3nnell rod shaped 1000 x - around 1-2 micrometers long gative grom should be pink ich…

A: Gram Staining It is a differential bacterial staining techniques used to differentiate Bacteria…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: Gram staining is the most common initial test of unknown identification, explain in your own words…

A: The Gram staining is a typical process that is used to separate two groups of microorganisms based…

Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used

A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…

Q: The acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______.…

A: The staining procedures used to differentiate the types of bacteria are known as a differential…

Q: The counterstain in the Gram stain is: A basic dye A negative stain A mordant An acid dye Necessary…

A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Histology, as well…

Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: two different

A: Gram staining- A test that checks for bacteria at the site of a suspected infection such as the…

Q: Additional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain,…

A: Staining is performed to enhance the sample present on a slide against its backdrop in such a way…

Q: A bacterial sample collected from a patient is processed using a Gram's stain. After staining, the…

A: Gram stain is a test that looks for bacteria in areas where an infection is suspected, such as the…

Q: Based from the results obtained from Gram staining, explain why bacterial cells retained or changed…

A: Staining is a technique that is used to enhance the contrast of the microscopic image through the…

Q: What is the purpose of iodine in the Gram stain?   To act as a counterstain   None of the other…

A: There are various staining techniques that are used to stain different cellular structures. The Gram…

Q: You mistakenly confused the primary stain and counter stain with crystal violet, how does you…

A: Gram staining is a widely used laboratory technique in microbiology and bacteriology to…

Q: You and a partner conduct the acid-fast staining using the same two bacteria. You know that there…

A: Acid-fast staining is a differential staining technique that proves to be an important diagnostic…

Q: Materials: .Bacterial cultures: E. coli & Staphylococcus aureus • Crystal violet (primary stain)…

A: Introduction:- Staining is the biochemical technique for coloring the specimens. There are different…

Q: Explain the relationship between bacterial cell wall with gram-positive and gram-negative in the…

A: Gram staining is the standard laboratory practice for distinguishing between two broad species of…

Q: please label this in the correct order for gram staining.  crystal violet, iodine, alcohol, and…

A: Gram staining is a method of staining useful for identifying and classifying bacteria into two major…

Q: Why don't you heatfix a negative stain slide?

A: Negative staining is used to investigate the physical structure, size, and organization of…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.